Examination of lactococcal bacteriophage c2 DNA replication using two-dimensional agarose gel electrophoresis

Michael J. Callanan; Paul W. O'Toole; Mark W. Lubbers; Kayla M. Polzin

doi:10.1016/S0378-1119(01)00702-8

Examination of lactococcal bacteriophage c2 DNA replication using two-dimensional agarose gel electrophoresis

Michael J. Callanan
, Paul W. O'Toole
, Mark W. Lubbers
, Kayla M. Polzin

Research output: Contribution to journal › Article › peer-review

Abstract

The ori locus of the prolate-headed lactococcal bacteriophage c2 supports plasmid replication in Lactococcus lactis in the absence of phage infection. To determine whether phage c2 DNA replication is initiated at the ori locus in vivo and to investigate the mechanism of phage DNA replication, replicating intermediates of phage c2 were analyzed using neutral/neutral two-dimensional agarose gel electrophoresis (2D). The 2D data revealed that c2 replicates via a theta mechanism and localized the initiation of theta replication to the ori region of the c2 genome.

Original language	English
Pages (from-to)	101-106
Number of pages	6
Journal	Gene
Volume	278
Issue number	1-2
DOIs	https://doi.org/10.1016/S0378-1119(01)00702-8
Publication status	Published - 31 Oct 2001
Externally published	Yes

Keywords

Bacteriophage
Lactococcus
Origin of replication
Theta replication

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10.1016/S0378-1119(01)00702-8

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title = "Examination of lactococcal bacteriophage c2 DNA replication using two-dimensional agarose gel electrophoresis",

abstract = "The ori locus of the prolate-headed lactococcal bacteriophage c2 supports plasmid replication in Lactococcus lactis in the absence of phage infection. To determine whether phage c2 DNA replication is initiated at the ori locus in vivo and to investigate the mechanism of phage DNA replication, replicating intermediates of phage c2 were analyzed using neutral/neutral two-dimensional agarose gel electrophoresis (2D). The 2D data revealed that c2 replicates via a theta mechanism and localized the initiation of theta replication to the ori region of the c2 genome.",

keywords = "Bacteriophage, Lactococcus, Origin of replication, Theta replication",

author = "Callanan, \{Michael J.\} and O'Toole, \{Paul W.\} and Lubbers, \{Mark W.\} and Polzin, \{Kayla M.\}",

year = "2001",

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doi = "10.1016/S0378-1119(01)00702-8",

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AU - Callanan, Michael J.

AU - O'Toole, Paul W.

AU - Lubbers, Mark W.

AU - Polzin, Kayla M.

PY - 2001/10/31

Y1 - 2001/10/31

N2 - The ori locus of the prolate-headed lactococcal bacteriophage c2 supports plasmid replication in Lactococcus lactis in the absence of phage infection. To determine whether phage c2 DNA replication is initiated at the ori locus in vivo and to investigate the mechanism of phage DNA replication, replicating intermediates of phage c2 were analyzed using neutral/neutral two-dimensional agarose gel electrophoresis (2D). The 2D data revealed that c2 replicates via a theta mechanism and localized the initiation of theta replication to the ori region of the c2 genome.

AB - The ori locus of the prolate-headed lactococcal bacteriophage c2 supports plasmid replication in Lactococcus lactis in the absence of phage infection. To determine whether phage c2 DNA replication is initiated at the ori locus in vivo and to investigate the mechanism of phage DNA replication, replicating intermediates of phage c2 were analyzed using neutral/neutral two-dimensional agarose gel electrophoresis (2D). The 2D data revealed that c2 replicates via a theta mechanism and localized the initiation of theta replication to the ori region of the c2 genome.

KW - Bacteriophage

KW - Lactococcus

KW - Origin of replication

KW - Theta replication

UR - https://www.scopus.com/pages/publications/0035980621

U2 - 10.1016/S0378-1119(01)00702-8

DO - 10.1016/S0378-1119(01)00702-8

M3 - Article

C2 - 11707326

AN - SCOPUS:0035980621

SN - 0378-1119

VL - 278

SP - 101

EP - 106

JO - Gene

JF - Gene

IS - 1-2

ER -

Examination of lactococcal bacteriophage c2 DNA replication using two-dimensional agarose gel electrophoresis

Abstract

Keywords

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