Fluorescence spectroscopy study of protoporphyrin IX in tissue-like phantoms

Fluorescence spectroscopy has been extensively investigated for disease diagnosis. In this framework, optical tissue phantoms are widely used for validating the biomedical device system in laboratory environment outside clinical procedures. Moreover, it is fundamental to consider that there are several scattering components and chromophores inside biological tissues and the interplay between scattering effects and absorption can result in a distortion of the emitted fluorescence signal. In this work, the photophysical behaviour of a set of liquid tissue like phantoms containing different compositions was analysed: phosphate buffer saline (PBS) was used as background medium, low fat milk as a scatterer, India ink as an absorber and PpIX dissolved in dimethyl formamide (DMF) as fluorophore. We examined the collected data in terms of the impact of surfactant Tween-20 on the background medium, scattering effects and combination of scattering and absorption within a luminescent body on PpIX. The results indicated that the intrinsic emission peaks are red-shifted by the scattering particles or surfactant, whilst the scatterer and absorber can alter the emission intensity substantially. We corroborated that phantoms containing higher surfactant content (< 0.5% Tween 20) are essential to prepare a stable aqueous phantoms.