Abstract
Artificial Insemination (AI) in goats is less developed compared to the large animals due to the lack of suit-able protocol of goat semen freezing and AI in India. The AI plays a pivotal role in the long-term ex-situ in vitro conservation of threatened breeds, increased productivity, and performance of a large number of non-descript and low potential goats. The objective of the present study was to enhance the life and fertility po-tential of cryopreserved semen and consequently the conception rate through frozen semen AI by the addi-tion of catalase in an extender. Ejaculates (30) were collected and were extended with tris-citric acid-fructose diluent. Catalases were added in diluent (0 IU/mL, 200 IU/mL, 400 IU/mL, 600 IU/mL, 800 IU/mL and 1000 IU/mL). Analysis of data using SPSS 16 revealed that motility, live sperm count, acro-somal integrity, and hypo-osmotic swelling positive spermatozoa were counted differed significantly (P<0.05) at different concentrations of catalase. The post-thaw motility, live sperm count, acrosomal integ-rity, and hypo-osmotic swelling positive spermatozoa were significantly (P<0.05) highest in 800 U/mL of catalase used in the present study. Mitochondrial membrane potential was also significantly highest (P<0.05) in frozen semen in 800 IU/mL catalase. Goats (20) were inseminated with frozen semen straw of 800 IU/mL catalase and 8 goats (40%) were pregnant. The conception rate in the control group was 35%.
| Original language | English |
|---|---|
| Pages (from-to) | 587-593 |
| Number of pages | 7 |
| Journal | Iranian Journal of Applied Animal Science |
| Volume | 11 |
| Issue number | 3 |
| Publication status | Published - 2021 |
| Externally published | Yes |
Keywords
- antioxidant
- catalase
- goat
- mitochondrial membrane potential
- post thaw semen