Generation of human induced pluripotent stem cell lines (hiPSC) from one bipolar disorder patient carrier of a DGKH risk haplotype and one non-risk-variant-carrier bipolar disorder patient

  • Viola Stella Palladino
  • , Nadia Omega Cipta Subrata
  • , Andreas Geburtig-Chiocchetti
  • , Rhiannon McNeill
  • , Per Hoffmann
  • , Andreas Reif
  • , Sarah Kittel-Schneider

Research output: Contribution to journalArticlepeer-review

Abstract

Fibroblasts were isolated from skin biopsies from two patients with bipolar I disorder. One patient was a 26 year old female carrying a risk haplotype in the DGKH (diacylglycerol kinase eta) gene and the other was a non-carrier 27 year old male. Patient fibroblasts were reprogrammed into human induced pluripotent stem cells (hiPSCs) by using a Sendai virus vector. DGKH-risk haplotype and non-risk haplotype hiPSCs showed expression of pluripotency markers and were able to differentiate into cells of the three germ layers. These cell models are useful to investigate the role of risk gene variants in bipolar disorder. Resource table.

Original languageEnglish
Pages (from-to)104-109
Number of pages6
JournalStem Cell Research
Volume32
DOIs
Publication statusPublished - Oct 2018
Externally publishedYes

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