@inbook{659bdc4bce9f4d50a0e213a783524b08,
title = "Generation of nonpolar deletion mutants in listeria monocytogenes using the “soeing” method",
abstract = "The ability to manipulate chromosomally encoded genes is a fundamental biological tool for the analysis of gene function. Here, we provide in greater depth a protocol for the creation of nonpolar unlabelled gene deletions in Listeria monocytogenes that are facilitated by the splicing overlap extension PCR technique. For mutagenesis in L. monocytogenes, we describe the pKSV7 plasmid-based approach, which facilitates the introduction of a spliced amplicon in place of the corresponding segment of chromosomal DNA.",
keywords = "Listeria monocytogenes, Nonpolar deletion mutants, pKSV7 vector, SOEing method",
author = "Kathrin Rychli and Eva Wagner and Guinane, \{Caitriona M.\} and Karen Daly and Colin Hill and Cotter, \{Paul D.\}",
note = "Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-0982-8\_13",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "165--175",
booktitle = "Methods in Molecular Biology",
}