TY - JOUR
T1 - Genetic characterization of the Bifidobacterium breve UCC 2003 hrcA locus
AU - Ventura, Marco
AU - Canchaya, Carlos
AU - Bernini, Valentina
AU - Del Casale, Antonio
AU - Dellaglio, Franco
AU - Neviani, Erasmo
AU - Fitzgerald, Gerald F.
AU - Van Sinderen, Douwe
PY - 2005/12
Y1 - 2005/12
N2 - The bacterial heat shock response is characterized by the elevated expression of a number of chaperone complexes and transcriptional regulators, including the DnaJ and the HrcA proteins. Genome analysis of Bifidobacterium breve UCC 2003 revealed a second copy of a dnaJ gene, named dnaJ2, which is flanked by the hrcA gene in a genetic constellation that appears to be unique to the actinobacteria. Phylogenetic analysis using 53 bacterial dnaJ sequences, including both dnaJ1 and dnaJ2 sequences, suggests that these genes have followed a different evolutionary development. Furthermore, the B. breve UCC 2003 dnaJ2 gene seems to be regulated in a manner that is different from that of the previously characterized dnaJ1 gene. The dnaJ2 gene, which was shown to be part of a 2.3-kb bicistronic operon with hrcA, was induced by osmotic shock but not significantly by heat stress. This induction pattern is unlike those of other characterized dnaJ genes and may be indicative of a unique stress adaptation strategy by this commensal microorganism.
AB - The bacterial heat shock response is characterized by the elevated expression of a number of chaperone complexes and transcriptional regulators, including the DnaJ and the HrcA proteins. Genome analysis of Bifidobacterium breve UCC 2003 revealed a second copy of a dnaJ gene, named dnaJ2, which is flanked by the hrcA gene in a genetic constellation that appears to be unique to the actinobacteria. Phylogenetic analysis using 53 bacterial dnaJ sequences, including both dnaJ1 and dnaJ2 sequences, suggests that these genes have followed a different evolutionary development. Furthermore, the B. breve UCC 2003 dnaJ2 gene seems to be regulated in a manner that is different from that of the previously characterized dnaJ1 gene. The dnaJ2 gene, which was shown to be part of a 2.3-kb bicistronic operon with hrcA, was induced by osmotic shock but not significantly by heat stress. This induction pattern is unlike those of other characterized dnaJ genes and may be indicative of a unique stress adaptation strategy by this commensal microorganism.
UR - https://www.scopus.com/pages/publications/29144479366
U2 - 10.1128/AEM.71.12.8998-9007.2005
DO - 10.1128/AEM.71.12.8998-9007.2005
M3 - Article
C2 - 16332909
AN - SCOPUS:29144479366
SN - 0099-2240
VL - 71
SP - 8998
EP - 9007
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 12
ER -