TY - JOUR
T1 - Genome analysis and characterisation of the exopolysaccharide produced by bifidobacterium longum subsp. Longum 35624™
AU - Altmann, Friedrich
AU - Kosma, Paul
AU - O'Callaghan, Amy
AU - Leahy, Sinead
AU - Bottacini, Francesca
AU - Molloy, Evelyn
AU - Plattner, Stephan
AU - Schiavi, Elisa
AU - Gleinser, Marita
AU - Groeger, David
AU - Grant, Ray
AU - Perez, Noelia Rodriguez
AU - Healy, Selena
AU - Svehla, Elisabeth
AU - Windwarder, Markus
AU - Hofinger, Andreas
AU - Motherway, Mary O.Connell
AU - Akdis, Cezmi A.
AU - Xu, Jun
AU - Roper, Jennifer
AU - Van Sinderen, Douwe
AU - O'Mahony, Liam
N1 - Publisher Copyright:
© 2016 Altmann et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2016/9
Y1 - 2016/9
N2 - The Bifibobacterium longum subsp. longum 35624™ strain (formerly named Bifidobacterium longum subsp. infantis) is a well described probiotic with clinical efficacy in Irritable Bowel Syndrome clinical trials and induces immunoregulatory effects in mice and in humans. This paper presents (a) the genome sequence of the organism allowing the assignment to its correct subspeciation longum; (b) a comparative genome assessment with other B. longum strains and (c) the molecular structure of the 35624 exopolysaccharide (EPS624). Comparative genome analysis of the 35624 strain with other B. longum strains determined that the sub-speciation of the strain is longum and revealed the presence of a 35624-specific gene cluster, predicted to encode the biosynthetic machinery for EPS624. Following isolation and acid treatment of the EPS, its chemical structure was determined using gas and liquid chromatography for sugar constituent and linkage analysis, electrospray and matrix assisted laser desorption ionization mass spectrometry for sequencing and NMR. The EPS consists of a branched hexasaccharide repeating unit containing two galactose and two glucose moieties, galacturonic acid and the unusual sugar 6-deoxy-L-talose. These data demonstrate that the B. longum 35624 strain has specific genetic features, one of which leads to the generation of a characteristic exopolysaccharide.
AB - The Bifibobacterium longum subsp. longum 35624™ strain (formerly named Bifidobacterium longum subsp. infantis) is a well described probiotic with clinical efficacy in Irritable Bowel Syndrome clinical trials and induces immunoregulatory effects in mice and in humans. This paper presents (a) the genome sequence of the organism allowing the assignment to its correct subspeciation longum; (b) a comparative genome assessment with other B. longum strains and (c) the molecular structure of the 35624 exopolysaccharide (EPS624). Comparative genome analysis of the 35624 strain with other B. longum strains determined that the sub-speciation of the strain is longum and revealed the presence of a 35624-specific gene cluster, predicted to encode the biosynthetic machinery for EPS624. Following isolation and acid treatment of the EPS, its chemical structure was determined using gas and liquid chromatography for sugar constituent and linkage analysis, electrospray and matrix assisted laser desorption ionization mass spectrometry for sequencing and NMR. The EPS consists of a branched hexasaccharide repeating unit containing two galactose and two glucose moieties, galacturonic acid and the unusual sugar 6-deoxy-L-talose. These data demonstrate that the B. longum 35624 strain has specific genetic features, one of which leads to the generation of a characteristic exopolysaccharide.
UR - https://www.scopus.com/pages/publications/84992183995
U2 - 10.1371/journal.pone.0162983
DO - 10.1371/journal.pone.0162983
M3 - Article
AN - SCOPUS:84992183995
SN - 1932-6203
VL - 11
JO - PLOS ONE
JF - PLOS ONE
IS - 9
M1 - e0162983
ER -