Genome analysis of Bifidobacterium bifidum PRL2010 reveals metabolic pathways for host-derived glycan foraging

  • Francesca Turroni
  • , Francesca Bottacini
  • , Elena Foroni
  • , Imke Mulder
  • , Jae Han Kim
  • , Aldert Zomer
  • , Borja Sánchez
  • , Alessandro Bidossi
  • , Alberto Ferrarini
  • , Vanessa Giubellini
  • , Massimo Delledonne
  • , Bernard Henrissat
  • , Pedro Coutinho
  • , Marco Oggioni
  • , Gerald F. Fitzgerald
  • , David Mills
  • , Abelardo Margolles
  • , Denise Kelly
  • , Douwe Van Sinderen
  • , Marco Ventura

Research output: Contribution to journalArticlepeer-review

Abstract

The human intestine is densely populated by a microbial consortium whose metabolic activities are influenced by, among others, bifidobacteria. However, the genetic basis of adaptation of bifidobacteria to the human gut is poorly understood. Analysis of the 2,214,650-bp genome of Bifidobacterium bifidum PRL2010, a strain isolated from infant stool, revealed a nutrient-acquisition strategy that targets host-derived glycans, such as those present in mucin. Proteome and transcriptome profiling revealed a set of chromosomal loci responsible for mucin metabolism that appear to be under common transcriptional control and with predicted functions that allow degradation of various O-linked glycans in mucin. Conservation of the latter gene clusters in various B. bifidum strains supports the notion that host-derived glycan catabolism is an important colonization factor for B. bifidum with concomitant impact on intestinal microbiota ecology.

Original languageEnglish
Pages (from-to)19514-19519
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number45
DOIs
Publication statusPublished - 9 Nov 2010

Keywords

  • Coevolution
  • Genomics
  • Host-glycans metabolism
  • Human gut intestinal bacteria
  • Mucin

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