TY - JOUR
T1 - How to feed the Mammalian gut microbiota
T2 - Bacterial and metabolic modulation by dietary fibers
AU - Ferrario, Chiara
AU - Statello, Rosario
AU - Carnevali, Luca
AU - Mancabelli, Leonardo
AU - Milani, Christian
AU - Mangifesta, Marta
AU - Duranti, Sabrina
AU - Lugli, Gabriele A.
AU - Jimenez, Beatriz
AU - Lodge, Samantha
AU - Viappiani, Alice
AU - Alessandri, Giulia
AU - Dall'Asta, Margerita
AU - Rio, Daniele Del
AU - Sgoifo, Andrea
AU - van Sinderen, Douwe
AU - Ventura, Marco
AU - Turroni, Francesca
N1 - Publisher Copyright:
© 2017 Ferrario, Statello, Carnevali, Mancabelli, Milani, Mangifesta, Duranti, Lugli, Jimenez, Lodge, Viappiani, Alessandri, Dall'Asta, Del Rio, Sgoifo, van Sinderen, Ventura and Turroni.
PY - 2017/9/12
Y1 - 2017/9/12
N2 - The composition of the gut microbiota of mammals is greatly influenced by diet. Therefore, evaluation of different food ingredients that may promote changes in the gut microbiota composition is an attractive approach to treat microbiota disturbances. In this study, three dietary fibers, such as inulin (I, 10%), resistant starch (RS, 10%), and citrus pectin (3%), were employed as supplements to normal chow diet of adult male rats for 2 weeks. Fecal microbiota composition and corresponding metabolite profiles were assessed before and after prebiotics supplementation. A general increase in the Bacteroidetes phylum was detected with a concurrent reduction in Firmicutes, in particular for I and RS experiments, while additional changes in the microbiota composition were evident at lower taxonomic levels for all the three substrates. Such modifications in the microbiota composition were correlated with changes in metabolic profiles of animals, in particular changes in acetate and succinate levels. This study represents a first attempt to modulate selectively the abundance and/or metabolic activity of various members of the gut microbiota by means of dietary fiber.
AB - The composition of the gut microbiota of mammals is greatly influenced by diet. Therefore, evaluation of different food ingredients that may promote changes in the gut microbiota composition is an attractive approach to treat microbiota disturbances. In this study, three dietary fibers, such as inulin (I, 10%), resistant starch (RS, 10%), and citrus pectin (3%), were employed as supplements to normal chow diet of adult male rats for 2 weeks. Fecal microbiota composition and corresponding metabolite profiles were assessed before and after prebiotics supplementation. A general increase in the Bacteroidetes phylum was detected with a concurrent reduction in Firmicutes, in particular for I and RS experiments, while additional changes in the microbiota composition were evident at lower taxonomic levels for all the three substrates. Such modifications in the microbiota composition were correlated with changes in metabolic profiles of animals, in particular changes in acetate and succinate levels. This study represents a first attempt to modulate selectively the abundance and/or metabolic activity of various members of the gut microbiota by means of dietary fiber.
KW - Acetate
KW - Diet
KW - Dietary fibers
KW - Microbiota
KW - Rat model
KW - Succinate
UR - https://www.scopus.com/pages/publications/85029637903
U2 - 10.3389/fmicb.2017.01749
DO - 10.3389/fmicb.2017.01749
M3 - Article
AN - SCOPUS:85029637903
SN - 1664-302X
VL - 8
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
IS - SEP
M1 - 1749
ER -