Human breastmilk-derived Bifidobacterium longum subsp. infantis CCFM1269 regulates bone formation by the GH/IGF axis through PI3K/AKT pathway

Mengfan Ding; Bowen Li; Haiqin Chen; Dong Liang; R. Paul Ross; Catherine Stanton; Jianxin Zhao; Wei Chen; Bo Yang

doi:10.1080/19490976.2023.2290344

Human breastmilk-derived Bifidobacterium longum subsp. infantis CCFM1269 regulates bone formation by the GH/IGF axis through PI3K/AKT pathway

Mengfan Ding
, Bowen Li
, Haiqin Chen
, Dong Liang
, R. Paul Ross
, Catherine Stanton
, Jianxin Zhao
, Wei Chen
, Bo Yang

APC Microbiome Ireland

Jiangnan University
China National Center for Food Safety Risk Assessment
Teagasc - Irish Agriculture and Food Development Authority

Research output: Contribution to journal › Article › peer-review

Abstract

Bifidobacterium longum subsp. infantis is a prevalent member of the gut microbiota of breastfed infants. In this study, the effects of human breastmilk-derived B.longum subsp. infantis CCFM1269 on bone formation in developing BALB/c mice were investigated. Newborn female and male mice were assigned to control group (administered saline), CCFM11269 group (administered B. longum subsp. infantis CCFM1269, 1 × 10₉ CFU/mouse/day) and I5TI group (administered B. longum subsp. infantis I5TI, 1 × 10₉ CFU/mouse/day) from 1-week-old to 3-, 4- and 5-week old. B. longum subsp. infantis I5TI served as a negative control in this study. The results demonstrated that B. longum subsp. infantis CCFM1269 promoted bone formation in growing mice by modulating the composition of the gut microbiota and metabolites. The expression of genes and proteins in the PI3K/AKT pathway was stimulated by B. longum subsp. infantis CCFM1269 through the GH/IGF-1 axis in growing mice. This finding suggests B. longum subsp. infantis CCFM1269 may be useful for modulating bone metabolism during growth.

Original language	English
Article number	2290344
Journal	Gut Microbes
Volume	16
Issue number	1
DOIs	https://doi.org/10.1080/19490976.2023.2290344
Publication status	Published - 2024

Keywords

B.Longum subsp. infantis
bone formation
GH/IGF axis
PI3K/AKT pathway

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10.1080/19490976.2023.2290344

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@article{366d2869837f49ae922d34c391417f89,

title = "Human breastmilk-derived Bifidobacterium longum subsp. infantis CCFM1269 regulates bone formation by the GH/IGF axis through PI3K/AKT pathway",

abstract = "Bifidobacterium longum subsp. infantis is a prevalent member of the gut microbiota of breastfed infants. In this study, the effects of human breastmilk-derived B.longum subsp. infantis CCFM1269 on bone formation in developing BALB/c mice were investigated. Newborn female and male mice were assigned to control group (administered saline), CCFM11269 group (administered B. longum subsp. infantis CCFM1269, 1 {\texttimes} 109 CFU/mouse/day) and I5TI group (administered B. longum subsp. infantis I5TI, 1 {\texttimes} 109 CFU/mouse/day) from 1-week-old to 3-, 4- and 5-week old. B. longum subsp. infantis I5TI served as a negative control in this study. The results demonstrated that B. longum subsp. infantis CCFM1269 promoted bone formation in growing mice by modulating the composition of the gut microbiota and metabolites. The expression of genes and proteins in the PI3K/AKT pathway was stimulated by B. longum subsp. infantis CCFM1269 through the GH/IGF-1 axis in growing mice. This finding suggests B. longum subsp. infantis CCFM1269 may be useful for modulating bone metabolism during growth.",

keywords = "B.Longum subsp. infantis, bone formation, GH/IGF axis, PI3K/AKT pathway",

author = "Mengfan Ding and Bowen Li and Haiqin Chen and Dong Liang and Ross, \{R. Paul\} and Catherine Stanton and Jianxin Zhao and Wei Chen and Bo Yang",

note = "Publisher Copyright: {\textcopyright} 2023 The Author(s). Published with license by Taylor \& Francis Group, LLC.",

year = "2024",

doi = "10.1080/19490976.2023.2290344",

language = "English",

volume = "16",

journal = "Gut Microbes",

issn = "1949-0976",

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T1 - Human breastmilk-derived Bifidobacterium longum subsp. infantis CCFM1269 regulates bone formation by the GH/IGF axis through PI3K/AKT pathway

AU - Ding, Mengfan

AU - Li, Bowen

AU - Chen, Haiqin

AU - Liang, Dong

AU - Ross, R. Paul

AU - Stanton, Catherine

AU - Zhao, Jianxin

AU - Chen, Wei

AU - Yang, Bo

PY - 2024

Y1 - 2024

N2 - Bifidobacterium longum subsp. infantis is a prevalent member of the gut microbiota of breastfed infants. In this study, the effects of human breastmilk-derived B.longum subsp. infantis CCFM1269 on bone formation in developing BALB/c mice were investigated. Newborn female and male mice were assigned to control group (administered saline), CCFM11269 group (administered B. longum subsp. infantis CCFM1269, 1 × 109 CFU/mouse/day) and I5TI group (administered B. longum subsp. infantis I5TI, 1 × 109 CFU/mouse/day) from 1-week-old to 3-, 4- and 5-week old. B. longum subsp. infantis I5TI served as a negative control in this study. The results demonstrated that B. longum subsp. infantis CCFM1269 promoted bone formation in growing mice by modulating the composition of the gut microbiota and metabolites. The expression of genes and proteins in the PI3K/AKT pathway was stimulated by B. longum subsp. infantis CCFM1269 through the GH/IGF-1 axis in growing mice. This finding suggests B. longum subsp. infantis CCFM1269 may be useful for modulating bone metabolism during growth.

AB - Bifidobacterium longum subsp. infantis is a prevalent member of the gut microbiota of breastfed infants. In this study, the effects of human breastmilk-derived B.longum subsp. infantis CCFM1269 on bone formation in developing BALB/c mice were investigated. Newborn female and male mice were assigned to control group (administered saline), CCFM11269 group (administered B. longum subsp. infantis CCFM1269, 1 × 109 CFU/mouse/day) and I5TI group (administered B. longum subsp. infantis I5TI, 1 × 109 CFU/mouse/day) from 1-week-old to 3-, 4- and 5-week old. B. longum subsp. infantis I5TI served as a negative control in this study. The results demonstrated that B. longum subsp. infantis CCFM1269 promoted bone formation in growing mice by modulating the composition of the gut microbiota and metabolites. The expression of genes and proteins in the PI3K/AKT pathway was stimulated by B. longum subsp. infantis CCFM1269 through the GH/IGF-1 axis in growing mice. This finding suggests B. longum subsp. infantis CCFM1269 may be useful for modulating bone metabolism during growth.

KW - B.Longum subsp. infantis

KW - bone formation

KW - GH/IGF axis

KW - PI3K/AKT pathway

UR - https://www.scopus.com/pages/publications/85180674679

U2 - 10.1080/19490976.2023.2290344

DO - 10.1080/19490976.2023.2290344

M3 - Article

AN - SCOPUS:85180674679

SN - 1949-0976

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JO - Gut Microbes

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M1 - 2290344

ER -

Human breastmilk-derived Bifidobacterium longum subsp. infantis CCFM1269 regulates bone formation by the GH/IGF axis through PI3K/AKT pathway

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Research from Jiangnan University Provide New Insights into Bifidobacterium (Human breastmilk-derived Bifidobacterium longum subsp. infantis CCFM1269 regulates bone formation by the GH/IGF axis through PI3K/AKT pathway)

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Human breastmilk-derived Bifidobacterium longum subsp. infantis CCFM1269 regulates bone formation by the GH/IGF axis through PI3K/AKT pathway

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Keywords

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Research from Jiangnan University Provide New Insights into Bifidobacterium (Human breastmilk-derived Bifidobacterium longum subsp. infantis CCFM1269 regulates bone formation by the GH/IGF axis through PI3K/AKT pathway)

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