Identification and characterization of an oleate hydratase-encoding gene from Bifidobacterium breve

Kerry Joan O'connell; Mary O.Connell Motherway; Alan A. Hennessey; Florian Brodhun; R. Paul Ross; Ivo Feussner; Catherine Stanton; Gerald F. Fitzgerald; Douwe Van Sinderen

doi:10.4161/bioe.24159

Identification and characterization of an oleate hydratase-encoding gene from Bifidobacterium breve

Kerry Joan O'connell
, Mary O.Connell Motherway
, Alan A. Hennessey
, Florian Brodhun
, R. Paul Ross
, Ivo Feussner
, Catherine Stanton
, Gerald F. Fitzgerald
, Douwe Van Sinderen

Teagasc - Irish Agriculture and Food Development Authority
University of Göttingen

Research output: Contribution to journal › Article › peer-review

Abstract

Bifidobacteria are common commensals of the mammalian gastrointestinal tract. Previous studies have suggested that a bifidobacterial myosin cross reactive antigen (MCRA) protein plays a role in bacterial stress tolerance, while this protein has also been linked to the biosynthesis of conjugated linoleic acid (CLA) in bifidobacteria. In order to increase our understanding on the role of MCRA in bifidobacteria we created and analyzed an insertion mutant of the MCRAencoding gene of B. breve NCFB 2258. Our results demonstrate that the MCRA protein of B. breve NCFB 2258 does not appear to play a role in CLA production, yet is an oleate hydratase, which contributes to bifidobacterial solvent stress protection.

Original language	English
Journal	Bioengineered Bugs
Volume	4
Issue number	5
DOIs	https://doi.org/10.4161/bioe.24159
Publication status	Published - Sep 2013

Keywords

Bifidobacteria
Conjugated linoleic acid
Hydratase
Myosin cross reactive antigen
Probiotic

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10.4161/bioe.24159

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title = "Identification and characterization of an oleate hydratase-encoding gene from Bifidobacterium breve",

abstract = "Bifidobacteria are common commensals of the mammalian gastrointestinal tract. Previous studies have suggested that a bifidobacterial myosin cross reactive antigen (MCRA) protein plays a role in bacterial stress tolerance, while this protein has also been linked to the biosynthesis of conjugated linoleic acid (CLA) in bifidobacteria. In order to increase our understanding on the role of MCRA in bifidobacteria we created and analyzed an insertion mutant of the MCRAencoding gene of B. breve NCFB 2258. Our results demonstrate that the MCRA protein of B. breve NCFB 2258 does not appear to play a role in CLA production, yet is an oleate hydratase, which contributes to bifidobacterial solvent stress protection.",

keywords = "Bifidobacteria, Conjugated linoleic acid, Hydratase, Myosin cross reactive antigen, Probiotic",

author = "O'connell, \{Kerry Joan\} and Motherway, \{Mary O.Connell\} and Hennessey, \{Alan A.\} and Florian Brodhun and Ross, \{R. Paul\} and Ivo Feussner and Catherine Stanton and Fitzgerald, \{Gerald F.\} and \{Van Sinderen\}, Douwe",

year = "2013",

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doi = "10.4161/bioe.24159",

language = "English",

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T1 - Identification and characterization of an oleate hydratase-encoding gene from Bifidobacterium breve

AU - O'connell, Kerry Joan

AU - Motherway, Mary O.Connell

AU - Hennessey, Alan A.

AU - Brodhun, Florian

AU - Ross, R. Paul

AU - Feussner, Ivo

AU - Stanton, Catherine

AU - Fitzgerald, Gerald F.

AU - Van Sinderen, Douwe

PY - 2013/9

Y1 - 2013/9

N2 - Bifidobacteria are common commensals of the mammalian gastrointestinal tract. Previous studies have suggested that a bifidobacterial myosin cross reactive antigen (MCRA) protein plays a role in bacterial stress tolerance, while this protein has also been linked to the biosynthesis of conjugated linoleic acid (CLA) in bifidobacteria. In order to increase our understanding on the role of MCRA in bifidobacteria we created and analyzed an insertion mutant of the MCRAencoding gene of B. breve NCFB 2258. Our results demonstrate that the MCRA protein of B. breve NCFB 2258 does not appear to play a role in CLA production, yet is an oleate hydratase, which contributes to bifidobacterial solvent stress protection.

AB - Bifidobacteria are common commensals of the mammalian gastrointestinal tract. Previous studies have suggested that a bifidobacterial myosin cross reactive antigen (MCRA) protein plays a role in bacterial stress tolerance, while this protein has also been linked to the biosynthesis of conjugated linoleic acid (CLA) in bifidobacteria. In order to increase our understanding on the role of MCRA in bifidobacteria we created and analyzed an insertion mutant of the MCRAencoding gene of B. breve NCFB 2258. Our results demonstrate that the MCRA protein of B. breve NCFB 2258 does not appear to play a role in CLA production, yet is an oleate hydratase, which contributes to bifidobacterial solvent stress protection.

KW - Bifidobacteria

KW - Conjugated linoleic acid

KW - Hydratase

KW - Myosin cross reactive antigen

KW - Probiotic

UR - https://www.scopus.com/pages/publications/84883345406

U2 - 10.4161/bioe.24159

DO - 10.4161/bioe.24159

M3 - Article

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AN - SCOPUS:84883345406

SN - 1949-1018

VL - 4

JO - Bioengineered Bugs

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IS - 5

ER -

Identification and characterization of an oleate hydratase-encoding gene from Bifidobacterium breve

Abstract

Keywords

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