@inbook{681facacf546467ea48ef217c13e94fb,
title = "Identification of DNA base modifications by means of pacific biosciences RS sequencing technology",
abstract = "Whole phage genomes can be sequenced readily using one or a combination of next generation sequencing (NGS) technologies. One of the most recently developed NGS platforms, the so-called Single-Molecule Real-Time (SMRT) sequencing approach provided by the PacBio RS platform, is particularly useful in providing complete (i.e., un-gapped) genome sequences, but differs from other technologies in that the platform also allows for downstream analysis to identify nucleotides that have been modified by DNA methylation. Here, we describe the methodological approach for the detection of genomic methylation motifs by means of SMRT sequencing.",
keywords = "Bacteriophage, Methylase, Methylome, PacBio, SMRT sequencing",
author = "Philip Kelleher and James Murphy and Jennifer Mahony and \{van Sinderen\}, Douwe",
note = "Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media LLC.",
year = "2018",
doi = "10.1007/978-1-4939-7343-9\_10",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "127--137",
booktitle = "Methods in Molecular Biology",
}