Imaging of oxygenation in 3D tissue models with multi-modal phosphorescent probes

Dmitri B. Papkovsky; Ruslan I. Dmitriev; Sergei Borisov

doi:10.1117/12.2079269

Imaging of oxygenation in 3D tissue models with multi-modal phosphorescent probes

Dmitri B. Papkovsky
, Ruslan I. Dmitriev
, Sergei Borisov

School of Biochemistry and Cell Biology

Research output: Chapter in Book/Report/Conference proceedings › Conference proceeding › peer-review

Abstract

Cell-penetrating phosphorescence based probes allow real-time, high-resolution imaging of O₂ concentration in respiring cells and 3D tissue models. We have developed a panel of such probes, small molecule and nanoparticle structures, which have different spectral characteristics, cell penetrating and tissue staining behavior. The probes are compatible with conventional live cell imaging platforms and can be used in different detection modalities, including ratiometric intensity and PLIM (Phosphorescence Lifetime IMaging) under one- or two-photon excitation. Analytical performance of these probes and utility of the O₂ imaging method have been demonstrated with different types of samples: 2D cell cultures, multi-cellular spheroids from cancer cell lines and primary neurons, excised slices from mouse brain, colon and bladder tissue, and live animals. They are particularly useful for hypoxia research, ex-vivo studies of tissue physiology, cell metabolism, cancer, inflammation, and multiplexing with many conventional fluorophors and markers of cellular function.

Original language	English
Title of host publication	Multiphoton Microscopy in the Biomedical Sciences XV
Editors	Karsten Konig, Peter T. C. So, Ammasi Periasamy
Publisher	SPIE
ISBN (Electronic)	9781628414196
DOIs	https://doi.org/10.1117/12.2079269
Publication status	Published - 2015
Event	Multiphoton Microscopy in the Biomedical Sciences XV - San Francisco, United States Duration: 8 Feb 2015 → 10 Feb 2015

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	9329
ISSN (Print)	1605-7422

Conference

Conference	Multiphoton Microscopy in the Biomedical Sciences XV
Country/Territory	United States
City	San Francisco
Period	8/02/15 → 10/02/15

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

3D tissue models
cell-penetrating probes
FLIM
fluorescence and phosphorescence lifetime imaging microscopy
imaging of tissue oxygenation
live cell imaging
Phosphorescent oxygen-sensitive probes

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10.1117/12.2079269

Cite this

Papkovsky, D. B., Dmitriev, R. I., & Borisov, S. (2015). Imaging of oxygenation in 3D tissue models with multi-modal phosphorescent probes. In K. Konig, P. T. C. So, & A. Periasamy (Eds.), Multiphoton Microscopy in the Biomedical Sciences XV Article 93290B (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9329). SPIE. https://doi.org/10.1117/12.2079269

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title = "Imaging of oxygenation in 3D tissue models with multi-modal phosphorescent probes",

abstract = "Cell-penetrating phosphorescence based probes allow real-time, high-resolution imaging of O2 concentration in respiring cells and 3D tissue models. We have developed a panel of such probes, small molecule and nanoparticle structures, which have different spectral characteristics, cell penetrating and tissue staining behavior. The probes are compatible with conventional live cell imaging platforms and can be used in different detection modalities, including ratiometric intensity and PLIM (Phosphorescence Lifetime IMaging) under one- or two-photon excitation. Analytical performance of these probes and utility of the O2 imaging method have been demonstrated with different types of samples: 2D cell cultures, multi-cellular spheroids from cancer cell lines and primary neurons, excised slices from mouse brain, colon and bladder tissue, and live animals. They are particularly useful for hypoxia research, ex-vivo studies of tissue physiology, cell metabolism, cancer, inflammation, and multiplexing with many conventional fluorophors and markers of cellular function.",

keywords = "3D tissue models, cell-penetrating probes, FLIM, fluorescence and phosphorescence lifetime imaging microscopy, imaging of tissue oxygenation, live cell imaging, Phosphorescent oxygen-sensitive probes",

author = "Papkovsky, \{Dmitri B.\} and Dmitriev, \{Ruslan I.\} and Sergei Borisov",

note = "Publisher Copyright: {\textcopyright} 2015 SPIE.; Multiphoton Microscopy in the Biomedical Sciences XV ; Conference date: 08-02-2015 Through 10-02-2015",

year = "2015",

doi = "10.1117/12.2079269",

language = "English",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "SPIE",

editor = "Karsten Konig and So, \{Peter T. C.\} and Ammasi Periasamy",

booktitle = "Multiphoton Microscopy in the Biomedical Sciences XV",

address = "United States",

}

Papkovsky, DB, Dmitriev, RI & Borisov, S 2015, Imaging of oxygenation in 3D tissue models with multi-modal phosphorescent probes. in K Konig, PTC So & A Periasamy (eds), Multiphoton Microscopy in the Biomedical Sciences XV., 93290B, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9329, SPIE, Multiphoton Microscopy in the Biomedical Sciences XV, San Francisco, United States, 8/02/15. https://doi.org/10.1117/12.2079269

Imaging of oxygenation in 3D tissue models with multi-modal phosphorescent probes. / Papkovsky, Dmitri B.; Dmitriev, Ruslan I.; Borisov, Sergei.
Multiphoton Microscopy in the Biomedical Sciences XV. ed. / Karsten Konig; Peter T. C. So; Ammasi Periasamy. SPIE, 2015. 93290B (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9329).

Research output: Chapter in Book/Report/Conference proceedings › Conference proceeding › peer-review

TY - GEN

T1 - Imaging of oxygenation in 3D tissue models with multi-modal phosphorescent probes

AU - Papkovsky, Dmitri B.

AU - Dmitriev, Ruslan I.

AU - Borisov, Sergei

PY - 2015

Y1 - 2015

N2 - Cell-penetrating phosphorescence based probes allow real-time, high-resolution imaging of O2 concentration in respiring cells and 3D tissue models. We have developed a panel of such probes, small molecule and nanoparticle structures, which have different spectral characteristics, cell penetrating and tissue staining behavior. The probes are compatible with conventional live cell imaging platforms and can be used in different detection modalities, including ratiometric intensity and PLIM (Phosphorescence Lifetime IMaging) under one- or two-photon excitation. Analytical performance of these probes and utility of the O2 imaging method have been demonstrated with different types of samples: 2D cell cultures, multi-cellular spheroids from cancer cell lines and primary neurons, excised slices from mouse brain, colon and bladder tissue, and live animals. They are particularly useful for hypoxia research, ex-vivo studies of tissue physiology, cell metabolism, cancer, inflammation, and multiplexing with many conventional fluorophors and markers of cellular function.

AB - Cell-penetrating phosphorescence based probes allow real-time, high-resolution imaging of O2 concentration in respiring cells and 3D tissue models. We have developed a panel of such probes, small molecule and nanoparticle structures, which have different spectral characteristics, cell penetrating and tissue staining behavior. The probes are compatible with conventional live cell imaging platforms and can be used in different detection modalities, including ratiometric intensity and PLIM (Phosphorescence Lifetime IMaging) under one- or two-photon excitation. Analytical performance of these probes and utility of the O2 imaging method have been demonstrated with different types of samples: 2D cell cultures, multi-cellular spheroids from cancer cell lines and primary neurons, excised slices from mouse brain, colon and bladder tissue, and live animals. They are particularly useful for hypoxia research, ex-vivo studies of tissue physiology, cell metabolism, cancer, inflammation, and multiplexing with many conventional fluorophors and markers of cellular function.

KW - 3D tissue models

KW - cell-penetrating probes

KW - FLIM

KW - fluorescence and phosphorescence lifetime imaging microscopy

KW - imaging of tissue oxygenation

KW - live cell imaging

KW - Phosphorescent oxygen-sensitive probes

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DO - 10.1117/12.2079269

M3 - Conference proceeding

AN - SCOPUS:84932137443

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Multiphoton Microscopy in the Biomedical Sciences XV

A2 - Konig, Karsten

A2 - So, Peter T. C.

A2 - Periasamy, Ammasi

PB - SPIE

T2 - Multiphoton Microscopy in the Biomedical Sciences XV

Y2 - 8 February 2015 through 10 February 2015

ER -

Imaging of oxygenation in 3D tissue models with multi-modal phosphorescent probes

Abstract

Publication series

Conference

UN SDGs

Keywords

Access to Document

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