TY - CHAP
T1 - Imaging of oxygenation in 3D tissue models with multi-modal phosphorescent probes
AU - Papkovsky, Dmitri B.
AU - Dmitriev, Ruslan I.
AU - Borisov, Sergei
N1 - Publisher Copyright:
© 2015 SPIE.
PY - 2015
Y1 - 2015
N2 - Cell-penetrating phosphorescence based probes allow real-time, high-resolution imaging of O2 concentration in respiring cells and 3D tissue models. We have developed a panel of such probes, small molecule and nanoparticle structures, which have different spectral characteristics, cell penetrating and tissue staining behavior. The probes are compatible with conventional live cell imaging platforms and can be used in different detection modalities, including ratiometric intensity and PLIM (Phosphorescence Lifetime IMaging) under one- or two-photon excitation. Analytical performance of these probes and utility of the O2 imaging method have been demonstrated with different types of samples: 2D cell cultures, multi-cellular spheroids from cancer cell lines and primary neurons, excised slices from mouse brain, colon and bladder tissue, and live animals. They are particularly useful for hypoxia research, ex-vivo studies of tissue physiology, cell metabolism, cancer, inflammation, and multiplexing with many conventional fluorophors and markers of cellular function.
AB - Cell-penetrating phosphorescence based probes allow real-time, high-resolution imaging of O2 concentration in respiring cells and 3D tissue models. We have developed a panel of such probes, small molecule and nanoparticle structures, which have different spectral characteristics, cell penetrating and tissue staining behavior. The probes are compatible with conventional live cell imaging platforms and can be used in different detection modalities, including ratiometric intensity and PLIM (Phosphorescence Lifetime IMaging) under one- or two-photon excitation. Analytical performance of these probes and utility of the O2 imaging method have been demonstrated with different types of samples: 2D cell cultures, multi-cellular spheroids from cancer cell lines and primary neurons, excised slices from mouse brain, colon and bladder tissue, and live animals. They are particularly useful for hypoxia research, ex-vivo studies of tissue physiology, cell metabolism, cancer, inflammation, and multiplexing with many conventional fluorophors and markers of cellular function.
KW - 3D tissue models
KW - cell-penetrating probes
KW - FLIM
KW - fluorescence and phosphorescence lifetime imaging microscopy
KW - imaging of tissue oxygenation
KW - live cell imaging
KW - Phosphorescent oxygen-sensitive probes
UR - https://www.scopus.com/pages/publications/84932137443
U2 - 10.1117/12.2079269
DO - 10.1117/12.2079269
M3 - Chapter
AN - SCOPUS:84932137443
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Multiphoton Microscopy in the Biomedical Sciences XV
A2 - Konig, Karsten
A2 - So, Peter T. C.
A2 - Periasamy, Ammasi
PB - SPIE
T2 - Multiphoton Microscopy in the Biomedical Sciences XV
Y2 - 8 February 2015 through 10 February 2015
ER -
