Improved luciferase tagging system for Listeria monocytogenes allows real-time monitoring in vivo and in vitro

C. U. Riedel; I. R. Monk; P. G. Casey; D. Morrissey; G. C. O'Sullivan; M. Tangney; C. Hill; C. G. Gahan

doi:10.1128/AEM.02940-06

Improved luciferase tagging system for Listeria monocytogenes allows real-time monitoring in vivo and in vitro

C. U. Riedel
, I. R. Monk
, P. G. Casey
, D. Morrissey
, G. C. O'Sullivan
, M. Tangney
, C. Hill
, C. G. Gahan

Research output: Contribution to journal › Article › peer-review

Abstract

An improved system for luciferase tagging Listeria monocytogenes was developed by constructing a highly active, constitutive promoter. This construct gave 100-fold-higher activity in broth than any native promoter tested and allowed for imaging of lux-tagged L. monocytogenes in food products, during murine infections, and in tumor targeting studies.

Original language	English
Pages (from-to)	3091-3094
Number of pages	4
Journal	Appl. Environ. Microbiol.
Volume	73
Issue number	9
DOIs	https://doi.org/10.1128/AEM.02940-06
Publication status	Published - 2007

Keywords

Animals Base Sequence Food Inspection/*methods *Food Microbiology Genetic Vectors/genetics Listeria monocytogenes/*metabolism Luciferases/*metabolism Luciferases, Bacterial Mice Molecular Sequence Data Operon/genetics Promoter Regions, Genetic/*genetics Sequence Analysis, DNA

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10.1128/AEM.02940-06

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title = "Improved luciferase tagging system for Listeria monocytogenes allows real-time monitoring in vivo and in vitro",

abstract = "An improved system for luciferase tagging Listeria monocytogenes was developed by constructing a highly active, constitutive promoter. This construct gave 100-fold-higher activity in broth than any native promoter tested and allowed for imaging of lux-tagged L. monocytogenes in food products, during murine infections, and in tumor targeting studies.",

keywords = "Animals Base Sequence Food Inspection/*methods *Food Microbiology Genetic Vectors/genetics Listeria monocytogenes/*metabolism Luciferases/*metabolism Luciferases, Bacterial Mice Molecular Sequence Data Operon/genetics Promoter Regions, Genetic/*genetics Sequence Analysis, DNA",

author = "Riedel, \{C. U.\} and Monk, \{I. R.\} and Casey, \{P. G.\} and D. Morrissey and O'Sullivan, \{G. C.\} and M. Tangney and C. Hill and Gahan, \{C. G.\}",

note = "Riedel, Christian U Monk, Ian R Casey, Pat G Morrissey, David O'Sullivan, Gerald C Tangney, Mark Hill, Colin Gahan, Cormac G M Comparative Study United States Applied and environmental microbiology Appl Environ Microbiol. 2007 May;73(9):3091-4. Epub 2007 Mar 9.",

year = "2007",

doi = "10.1128/AEM.02940-06",

language = "English",

volume = "73",

pages = "3091--3094",

journal = "Appl. Environ. Microbiol.",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "9",

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TY - JOUR

T1 - Improved luciferase tagging system for Listeria monocytogenes allows real-time monitoring in vivo and in vitro

AU - Riedel, C. U.

AU - Monk, I. R.

AU - Casey, P. G.

AU - Morrissey, D.

AU - O'Sullivan, G. C.

AU - Tangney, M.

AU - Hill, C.

AU - Gahan, C. G.

N1 - Riedel, Christian U Monk, Ian R Casey, Pat G Morrissey, David O'Sullivan, Gerald C Tangney, Mark Hill, Colin Gahan, Cormac G M Comparative Study United States Applied and environmental microbiology Appl Environ Microbiol. 2007 May;73(9):3091-4. Epub 2007 Mar 9.

PY - 2007

Y1 - 2007

N2 - An improved system for luciferase tagging Listeria monocytogenes was developed by constructing a highly active, constitutive promoter. This construct gave 100-fold-higher activity in broth than any native promoter tested and allowed for imaging of lux-tagged L. monocytogenes in food products, during murine infections, and in tumor targeting studies.

AB - An improved system for luciferase tagging Listeria monocytogenes was developed by constructing a highly active, constitutive promoter. This construct gave 100-fold-higher activity in broth than any native promoter tested and allowed for imaging of lux-tagged L. monocytogenes in food products, during murine infections, and in tumor targeting studies.

KW - Animals Base Sequence Food Inspection/methods Food Microbiology Genetic Vectors/genetics Listeria monocytogenes/metabolism Luciferases/metabolism Luciferases, Bacterial Mice Molecular Sequence Data Operon/genetics Promoter Regions, Genetic/genetics Sequen

U2 - 10.1128/AEM.02940-06

DO - 10.1128/AEM.02940-06

M3 - Article

SN - 0099-2240

VL - 73

SP - 3091

EP - 3094

JO - Appl. Environ. Microbiol.

JF - Appl. Environ. Microbiol.

IS - 9

ER -

Improved luciferase tagging system for Listeria monocytogenes allows real-time monitoring in vivo and in vitro

Abstract

Keywords

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