TY - JOUR
T1 - Influence of assessors' qualities and analytical conditions on gas chromatography-olfactometry analysis
AU - Van Ruth, Saskia M.
AU - O'Connor, Catherine H.
PY - 2001
Y1 - 2001
N2 - Gas chromatography-olfactometry is a commonly used technique for analysis of odour active compounds. The present study deals with the influence of methodological factors on gas chromatography-olfactometry data, i.e. assessors' qualities and analytical conditions. With respect to the assessors' qualities, the effects of training and fatigue/alertness were studied. Analytical conditions included concentration aspects resulting from varying chromatographic peak shapes and humidified air flow rates at the sniff port. The compounds 2-butanone, diacetyl, ethyl acetate, 3-methyl-1-butanol, ethyl butyrate, hexanal, 2-heptanone and α-pinene were analysed by gas chromatography-olfactometry using the detection frequency and posterior intensity method for collection and processing of the data. Training did not affect the detection of the odour active compounds, but it decreased noise levels considerably. No significant effect of fatigue or decreased alertness was found for the 45-min sessions. The data were influenced by extreme peak shape differences and an intensity optimum was observed for all compounds at a humidified air flow rate of 6 ml min-1. Assessors' qualities and analytical conditions were shown to affect GC-O data and require optimisation for sound gas chromatography-olfactometry analysis.
AB - Gas chromatography-olfactometry is a commonly used technique for analysis of odour active compounds. The present study deals with the influence of methodological factors on gas chromatography-olfactometry data, i.e. assessors' qualities and analytical conditions. With respect to the assessors' qualities, the effects of training and fatigue/alertness were studied. Analytical conditions included concentration aspects resulting from varying chromatographic peak shapes and humidified air flow rates at the sniff port. The compounds 2-butanone, diacetyl, ethyl acetate, 3-methyl-1-butanol, ethyl butyrate, hexanal, 2-heptanone and α-pinene were analysed by gas chromatography-olfactometry using the detection frequency and posterior intensity method for collection and processing of the data. Training did not affect the detection of the odour active compounds, but it decreased noise levels considerably. No significant effect of fatigue or decreased alertness was found for the 45-min sessions. The data were influenced by extreme peak shape differences and an intensity optimum was observed for all compounds at a humidified air flow rate of 6 ml min-1. Assessors' qualities and analytical conditions were shown to affect GC-O data and require optimisation for sound gas chromatography-olfactometry analysis.
KW - Aroma
KW - Flavour
KW - Gas chromatography-olfactometry
KW - Odour active compounds
KW - Volatiles
UR - https://www.scopus.com/pages/publications/0003163221
U2 - 10.1007/s002170100360
DO - 10.1007/s002170100360
M3 - Article
AN - SCOPUS:0003163221
SN - 1438-2377
VL - 213
SP - 77
EP - 82
JO - European Food Research and Technology
JF - European Food Research and Technology
IS - 1
ER -