TY - JOUR
T1 - Insertional inactivation of determinants for Mg2+ and Co 2+ transport as a tool for screening recombinant Lactococcus species clones
AU - Mills, Susan
AU - Coffey, Aidan
AU - Hill, Colin
AU - Fitzgerald, Gerald F.
AU - McAuliffe, Olivia
AU - Ross, R. Paul
PY - 2005/8
Y1 - 2005/8
N2 - Insertional inactivation of the plasmid-encoded determinants for Mg 2+ and Co2+ transport, orf18/corA, provides a tool for screening recombinant clones in Lactococcus, based on the observation that overexpression of orfl8/corA results in cell growth inhibition on certain concentrations of CoCl2. The lacticin 3147 immunity gene, ltnI, was used to insertionally inactivate orfl8/corA. The resulting clones were capable of growth on concentrations of CoCl2 that were inhibitory to the parent strain. Since only 3 of 17 lactococcal starters naturally harbor cor A, the system has potential as a screen for selecting recombinant lactococcal clones.
AB - Insertional inactivation of the plasmid-encoded determinants for Mg 2+ and Co2+ transport, orf18/corA, provides a tool for screening recombinant clones in Lactococcus, based on the observation that overexpression of orfl8/corA results in cell growth inhibition on certain concentrations of CoCl2. The lacticin 3147 immunity gene, ltnI, was used to insertionally inactivate orfl8/corA. The resulting clones were capable of growth on concentrations of CoCl2 that were inhibitory to the parent strain. Since only 3 of 17 lactococcal starters naturally harbor cor A, the system has potential as a screen for selecting recombinant lactococcal clones.
UR - https://www.scopus.com/pages/publications/23744458463
U2 - 10.1128/AEM.71.8.4897-4901.2005
DO - 10.1128/AEM.71.8.4897-4901.2005
M3 - Article
C2 - 16085892
AN - SCOPUS:23744458463
SN - 0099-2240
VL - 71
SP - 4897
EP - 4901
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 8
ER -