Interference of Mg2+ and Ca2+ on protein determination with Lowry's method

Qiushi Xie; Gavin M. Burnell

doi:10.1016/0305-0491(94)90192-9

Interference of Mg²⁺ and Ca²⁺ on protein determination with Lowry's method

Qiushi Xie
, Gavin M. Burnell

University College Cork

Research output: Contribution to journal › Article › peer-review

Abstract

The effect of Mg²⁺ (0.84-6.74 mM) and Ca²⁺ (0.18-1.45 mM) on the Lowry method was studied. Mg²⁺ and Ca²⁺ affected the absorbance of both the protein-free blank and protein samples. The tolerable limit was <0.84 mM for Mg²⁺ and 0.55 mM for Ca²⁺. The interference by Mg²⁺ was more significant than Ca²⁺ at the same concentration. A hypothesis was proposed in respect of the mechanism of interference by Mg²⁺: the Mg(OH)₂ precipitate binds with protein and some constituent of Lowry reagent B and thus reduces their sensitivity to Folin reagent.

Original language	English
Pages (from-to)	605-608
Number of pages	4
Journal	Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
Volume	107
Issue number	4
DOIs	https://doi.org/10.1016/0305-0491(94)90192-9
Publication status	Published - Apr 1994

Keywords

Interference of Mg
Lowry's method

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10.1016/0305-0491(94)90192-9

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title = "Interference of Mg2+ and Ca2+ on protein determination with Lowry's method",

abstract = "The effect of Mg2+ (0.84-6.74 mM) and Ca2+ (0.18-1.45 mM) on the Lowry method was studied. Mg2+ and Ca2+ affected the absorbance of both the protein-free blank and protein samples. The tolerable limit was <0.84 mM for Mg2+ and 0.55 mM for Ca2+. The interference by Mg2+ was more significant than Ca2+ at the same concentration. A hypothesis was proposed in respect of the mechanism of interference by Mg2+: the Mg(OH)2 precipitate binds with protein and some constituent of Lowry reagent B and thus reduces their sensitivity to Folin reagent.",

keywords = "Interference of Mg, Lowry's method",

author = "Qiushi Xie and Burnell, \{Gavin M.\}",

year = "1994",

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doi = "10.1016/0305-0491(94)90192-9",

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T1 - Interference of Mg2+ and Ca2+ on protein determination with Lowry's method

AU - Xie, Qiushi

AU - Burnell, Gavin M.

PY - 1994/4

Y1 - 1994/4

N2 - The effect of Mg2+ (0.84-6.74 mM) and Ca2+ (0.18-1.45 mM) on the Lowry method was studied. Mg2+ and Ca2+ affected the absorbance of both the protein-free blank and protein samples. The tolerable limit was <0.84 mM for Mg2+ and 0.55 mM for Ca2+. The interference by Mg2+ was more significant than Ca2+ at the same concentration. A hypothesis was proposed in respect of the mechanism of interference by Mg2+: the Mg(OH)2 precipitate binds with protein and some constituent of Lowry reagent B and thus reduces their sensitivity to Folin reagent.

AB - The effect of Mg2+ (0.84-6.74 mM) and Ca2+ (0.18-1.45 mM) on the Lowry method was studied. Mg2+ and Ca2+ affected the absorbance of both the protein-free blank and protein samples. The tolerable limit was <0.84 mM for Mg2+ and 0.55 mM for Ca2+. The interference by Mg2+ was more significant than Ca2+ at the same concentration. A hypothesis was proposed in respect of the mechanism of interference by Mg2+: the Mg(OH)2 precipitate binds with protein and some constituent of Lowry reagent B and thus reduces their sensitivity to Folin reagent.

KW - Interference of Mg

KW - Lowry's method

UR - https://www.scopus.com/pages/publications/0028178003

U2 - 10.1016/0305-0491(94)90192-9

DO - 10.1016/0305-0491(94)90192-9

M3 - Article

AN - SCOPUS:0028178003

SN - 1096-4959

VL - 107

SP - 605

EP - 608

JO - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology

JF - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology

IS - 4

ER -

Interference of Mg²⁺ and Ca²⁺ on protein determination with Lowry's method

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