Intracellular probes for imaging oxygen concentration: How good are they?

Ruslan I. Dmitriev; Dmitri B. Papkovsky

doi:10.1088/2050-6120/3/3/034001

Intracellular probes for imaging oxygen concentration: How good are they?

Ruslan I. Dmitriev
, Dmitri B. Papkovsky

School of Biochemistry and Cell Biology

Research output: Contribution to journal › Article › peer-review

Abstract

In the last decade a number of cell-permeable phosphorescence based probes for imaging of (intra) cellular oxygen (icO²) have been described. These small molecule, supramolecular and nanoparticle structures, although allowing analysis of hypoxia, local gradients and fluctuations in O², responses to stimulation and drug treatment at sub-cellular level with high spatial and temporal resolution, differ significantly in their operational performance and applicability to different cell and tissue models. Here we discuss and compare these probes with respect to their staining efficiency, brightness, photostability, toxicity, cell specificity, compatibility with different cell and tissue models, and analytical performance. Merits and limitations of particular probes are highlighted and strategies for development of new high-performance O2 imaging probes defined. Key application areas in hypoxia research, stem cells, cancer biology and tissue physiology are also discussed.

Original language	English
Article number	034001
Journal	Methods and Applications in Fluorescence
Volume	3
Issue number	3
DOIs	https://doi.org/10.1088/2050-6120/3/3/034001
Publication status	Published - Sep 2015

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

3D tissue models
FLIM
Hypoxia
Intracellular and cell-permeable probes
Phosphorescence quenching
Phosphorescent oxygen-sensitive probes

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10.1088/2050-6120/3/3/034001

Cite this

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title = "Intracellular probes for imaging oxygen concentration: How good are they?",

abstract = "In the last decade a number of cell-permeable phosphorescence based probes for imaging of (intra) cellular oxygen (icO2) have been described. These small molecule, supramolecular and nanoparticle structures, although allowing analysis of hypoxia, local gradients and fluctuations in O2, responses to stimulation and drug treatment at sub-cellular level with high spatial and temporal resolution, differ significantly in their operational performance and applicability to different cell and tissue models. Here we discuss and compare these probes with respect to their staining efficiency, brightness, photostability, toxicity, cell specificity, compatibility with different cell and tissue models, and analytical performance. Merits and limitations of particular probes are highlighted and strategies for development of new high-performance O2 imaging probes defined. Key application areas in hypoxia research, stem cells, cancer biology and tissue physiology are also discussed.",

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doi = "10.1088/2050-6120/3/3/034001",

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AU - Dmitriev, Ruslan I.

AU - Papkovsky, Dmitri B.

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N2 - In the last decade a number of cell-permeable phosphorescence based probes for imaging of (intra) cellular oxygen (icO2) have been described. These small molecule, supramolecular and nanoparticle structures, although allowing analysis of hypoxia, local gradients and fluctuations in O2, responses to stimulation and drug treatment at sub-cellular level with high spatial and temporal resolution, differ significantly in their operational performance and applicability to different cell and tissue models. Here we discuss and compare these probes with respect to their staining efficiency, brightness, photostability, toxicity, cell specificity, compatibility with different cell and tissue models, and analytical performance. Merits and limitations of particular probes are highlighted and strategies for development of new high-performance O2 imaging probes defined. Key application areas in hypoxia research, stem cells, cancer biology and tissue physiology are also discussed.

AB - In the last decade a number of cell-permeable phosphorescence based probes for imaging of (intra) cellular oxygen (icO2) have been described. These small molecule, supramolecular and nanoparticle structures, although allowing analysis of hypoxia, local gradients and fluctuations in O2, responses to stimulation and drug treatment at sub-cellular level with high spatial and temporal resolution, differ significantly in their operational performance and applicability to different cell and tissue models. Here we discuss and compare these probes with respect to their staining efficiency, brightness, photostability, toxicity, cell specificity, compatibility with different cell and tissue models, and analytical performance. Merits and limitations of particular probes are highlighted and strategies for development of new high-performance O2 imaging probes defined. Key application areas in hypoxia research, stem cells, cancer biology and tissue physiology are also discussed.

KW - 3D tissue models

KW - FLIM

KW - Hypoxia

KW - Intracellular and cell-permeable probes

KW - Phosphorescence quenching

KW - Phosphorescent oxygen-sensitive probes

UR - https://www.scopus.com/pages/publications/84974603095

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DO - 10.1088/2050-6120/3/3/034001

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SN - 2050-6120

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JO - Methods and Applications in Fluorescence

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Intracellular probes for imaging oxygen concentration: How good are they?

Abstract

UN SDGs

Keywords

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