Isolation and characterization of neural stem cells from caprine

Central nervous system (CNS) has no or very little capacity to self repair. Neural tissue transplantation is impractical because it is hard to find a brain donor, and because of the low survival rate of grafted neural cells. Therefore, the need for constructing a functional brain tissue is very critical. In this study neural stem cells were isolated and expanded from caprine fetal brain in adherent culture. After 4-5 days of culture there were formation of small colonies and after about 10 days cells become confluent. Cells from passage 1 to 4 were used in study. These neural stem cells were characterized with neural stem cell specific markers nestin (NES), PAX6 and SOX2 by PCR and immunocytochemistry. The cells were expressed neural stem cell markers nestin, pax 6 and Sox 2.