TY - CHAP
T1 - Isolation of Chromosomal and Plasmid DNA from Bifidobacteria
AU - Esteban-Torres, Maria
AU - Ruiz, Lorena
AU - Sanchez-Gallardo, Rocio
AU - van Sinderen, Douwe
N1 - Publisher Copyright:
© 2021, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2021
Y1 - 2021
N2 - Rapid and efficient protocols aimed at the isolation and purification of DNA for the purpose of downstream applications, such as cloning, PCR, Southern blotting, or sequencing, are essential for genetic, biochemical, and molecular biological analyses of a given bacterium. The protocols herein presented provide a robust and efficient method for the isolation of chromosomal and plasmid DNA from Bifidobacterium strains by organic extraction. The methods are simple, and the yield, purity, and quality of the DNA are adequate to perform various downstream applications including next-generation sequencing.
AB - Rapid and efficient protocols aimed at the isolation and purification of DNA for the purpose of downstream applications, such as cloning, PCR, Southern blotting, or sequencing, are essential for genetic, biochemical, and molecular biological analyses of a given bacterium. The protocols herein presented provide a robust and efficient method for the isolation of chromosomal and plasmid DNA from Bifidobacterium strains by organic extraction. The methods are simple, and the yield, purity, and quality of the DNA are adequate to perform various downstream applications including next-generation sequencing.
KW - Bifidobacteria
KW - Cell lysis
KW - DNA purification
KW - Plasmid isolation
KW - Whole genome sequencing
UR - https://www.scopus.com/pages/publications/85102134229
U2 - 10.1007/978-1-0716-1274-3_3
DO - 10.1007/978-1-0716-1274-3_3
M3 - Chapter
C2 - 33649945
AN - SCOPUS:85102134229
T3 - Methods in Molecular Biology
SP - 21
EP - 29
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -