Local bacteria affect the efficacy of chemotherapeutic drugs

Panos Lehouritis; Joanne Cummins; Michael Stanton; Carola T. Murphy; Florence O. McCarthy; Gregor Reid; Camilla Urbaniak; William L. Byrne; Mark Tangney

doi:10.1038/srep14554

Local bacteria affect the efficacy of chemotherapeutic drugs

Panos Lehouritis
, Joanne Cummins
, Michael Stanton
, Carola T. Murphy
, Florence O. McCarthy
, Gregor Reid
, Camilla Urbaniak
, William L. Byrne
, Mark Tangney

School of Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

In this study, the potential effects of bacteria on the efficacy of frequently used chemotherapies was examined. Bacteria and cancer cell lines were examined in vitro and in vivo for changes in the efficacy of cancer cell killing mediated by chemotherapeutic agents. Of 30 drugs examined in vitro, the efficacy of 10 was found to be significantly inhibited by certain bacteria, while the same bacteria improved the efficacy of six others. HPLC and mass spectrometry analyses of sample drugs (gemcitabine, fludarabine, cladribine, CB1954) demonstrated modification of drug chemical structure. The chemoresistance or increased cytotoxicity observed in vitro with sample drugs (gemcitabine and CB1954) was replicated in in vivo murine subcutaneous tumour models. These findings suggest that bacterial presence in the body due to systemic or local infection may influence tumour responses or off-target toxicity during chemotherapy.

Original language	English
Article number	14554
Journal	Scientific Reports
Volume	5
DOIs	https://doi.org/10.1038/srep14554
Publication status	Published - 29 Sep 2015

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10.1038/srep14554

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title = "Local bacteria affect the efficacy of chemotherapeutic drugs",

abstract = "In this study, the potential effects of bacteria on the efficacy of frequently used chemotherapies was examined. Bacteria and cancer cell lines were examined in vitro and in vivo for changes in the efficacy of cancer cell killing mediated by chemotherapeutic agents. Of 30 drugs examined in vitro, the efficacy of 10 was found to be significantly inhibited by certain bacteria, while the same bacteria improved the efficacy of six others. HPLC and mass spectrometry analyses of sample drugs (gemcitabine, fludarabine, cladribine, CB1954) demonstrated modification of drug chemical structure. The chemoresistance or increased cytotoxicity observed in vitro with sample drugs (gemcitabine and CB1954) was replicated in in vivo murine subcutaneous tumour models. These findings suggest that bacterial presence in the body due to systemic or local infection may influence tumour responses or off-target toxicity during chemotherapy.",

author = "Panos Lehouritis and Joanne Cummins and Michael Stanton and Murphy, \{Carola T.\} and McCarthy, \{Florence O.\} and Gregor Reid and Camilla Urbaniak and Byrne, \{William L.\} and Mark Tangney",

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doi = "10.1038/srep14554",

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T1 - Local bacteria affect the efficacy of chemotherapeutic drugs

AU - Lehouritis, Panos

AU - Cummins, Joanne

AU - Stanton, Michael

AU - Murphy, Carola T.

AU - McCarthy, Florence O.

AU - Reid, Gregor

AU - Urbaniak, Camilla

AU - Byrne, William L.

AU - Tangney, Mark

PY - 2015/9/29

Y1 - 2015/9/29

N2 - In this study, the potential effects of bacteria on the efficacy of frequently used chemotherapies was examined. Bacteria and cancer cell lines were examined in vitro and in vivo for changes in the efficacy of cancer cell killing mediated by chemotherapeutic agents. Of 30 drugs examined in vitro, the efficacy of 10 was found to be significantly inhibited by certain bacteria, while the same bacteria improved the efficacy of six others. HPLC and mass spectrometry analyses of sample drugs (gemcitabine, fludarabine, cladribine, CB1954) demonstrated modification of drug chemical structure. The chemoresistance or increased cytotoxicity observed in vitro with sample drugs (gemcitabine and CB1954) was replicated in in vivo murine subcutaneous tumour models. These findings suggest that bacterial presence in the body due to systemic or local infection may influence tumour responses or off-target toxicity during chemotherapy.

AB - In this study, the potential effects of bacteria on the efficacy of frequently used chemotherapies was examined. Bacteria and cancer cell lines were examined in vitro and in vivo for changes in the efficacy of cancer cell killing mediated by chemotherapeutic agents. Of 30 drugs examined in vitro, the efficacy of 10 was found to be significantly inhibited by certain bacteria, while the same bacteria improved the efficacy of six others. HPLC and mass spectrometry analyses of sample drugs (gemcitabine, fludarabine, cladribine, CB1954) demonstrated modification of drug chemical structure. The chemoresistance or increased cytotoxicity observed in vitro with sample drugs (gemcitabine and CB1954) was replicated in in vivo murine subcutaneous tumour models. These findings suggest that bacterial presence in the body due to systemic or local infection may influence tumour responses or off-target toxicity during chemotherapy.

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SN - 2045-2322

VL - 5

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JF - Scientific Reports

M1 - 14554

ER -

Local bacteria affect the efficacy of chemotherapeutic drugs

Abstract

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