TY - JOUR
T1 - Macrophage-dependent candidacidal mechanisms in the murine system
T2 - Comparison of murine kupffer cell and peritoneal macrophage candidacidal mechanisms
AU - Redmond, H. Paul
AU - Shou, Jian
AU - Gallagher, Hubert J.
AU - Kelly, Cathal J.
AU - Daly, John M.
PY - 1993
Y1 - 1993
N2 - Candida albicans infection is common in immunocompromised patients. The role of fixed tissue macrophages (Mφ), including Kupffer cells (KC) and peritoneal macrophages (PM7phi;), in host defense against C. albicans is unclear. This study examined murine Mφ candidacidal mechanisms and evaluated the in vitro role of the macrophage-activating factor IFN-γ in augmenting these mechanisms. The effect of in vivo administration of IFN-γ on survival aftrer lethal C. albicans challenge in the murine system was also assessed. Percent PMφ and KC ingestion of C. albicans were similar. Prior opsonization of Candida increased the percentage of Mφ ingestion of this pathogen. PMφ and KC phagocytic function was similar for both nonopsonized and opsonized C. albicans, but KC demonstrated markedly decreased ability to kill this pathogen (O2-, Candida killing). IFN-γ enhanced KC and PMφ candidacidal activity. PMφ and KC Ag presentation was increased in early Candida infection, but diminished in established infection, when the majority of animals died. C. albicans failed to elicit significant amounts of either IL-1 or TNF compared with LPS stimulation of PMφ and KC in vitro. IFN-γ treatment in vivo was associated with significantly improved survival (p < 0.01).
AB - Candida albicans infection is common in immunocompromised patients. The role of fixed tissue macrophages (Mφ), including Kupffer cells (KC) and peritoneal macrophages (PM7phi;), in host defense against C. albicans is unclear. This study examined murine Mφ candidacidal mechanisms and evaluated the in vitro role of the macrophage-activating factor IFN-γ in augmenting these mechanisms. The effect of in vivo administration of IFN-γ on survival aftrer lethal C. albicans challenge in the murine system was also assessed. Percent PMφ and KC ingestion of C. albicans were similar. Prior opsonization of Candida increased the percentage of Mφ ingestion of this pathogen. PMφ and KC phagocytic function was similar for both nonopsonized and opsonized C. albicans, but KC demonstrated markedly decreased ability to kill this pathogen (O2-, Candida killing). IFN-γ enhanced KC and PMφ candidacidal activity. PMφ and KC Ag presentation was increased in early Candida infection, but diminished in established infection, when the majority of animals died. C. albicans failed to elicit significant amounts of either IL-1 or TNF compared with LPS stimulation of PMφ and KC in vitro. IFN-γ treatment in vivo was associated with significantly improved survival (p < 0.01).
UR - https://www.scopus.com/pages/publications/0027159587
M3 - Article
C2 - 8385685
AN - SCOPUS:0027159587
SN - 0022-1767
VL - 150
SP - 3427
EP - 3433
JO - Journal of Immunology
JF - Journal of Immunology
IS - 8 PART 1
ER -