Mass spectrometry identification of age-associated proteins from the malaria mosquitoes Anopheles gambiae s.s. and Anopheles stephensi

Maggy T. Sikulu; James Monkman; Keyur A. Dave; Marcus L. Hastie; Patricia E. Dale; Roger L. Kitching; Gerry F. Killeen; Brian H. Kay; Jeffry J. Gorman; Leon E. Hugo

doi:10.1016/j.dib.2015.07.007

Mass spectrometry identification of age-associated proteins from the malaria mosquitoes Anopheles gambiae s.s. and Anopheles stephensi

Maggy T. Sikulu
, James Monkman
, Keyur A. Dave
, Marcus L. Hastie
, Patricia E. Dale
, Roger L. Kitching
, Gerry F. Killeen
, Brian H. Kay
, Jeffry J. Gorman
, Leon E. Hugo

Research output: Contribution to journal › Article › peer-review

Abstract

This study investigated proteomic changes occurring in Anopheles gambiae and Anopheles stephensi during adult mosquito aging. These changes were evaluated using two-dimensional difference gel electrophoresis (2D-DIGE) and the identities of aging related proteins were determined using capillary high-pressure liquid chromatography (capHPLC) coupled with a linear ion-trap (LTQ)-Orbitrap XL hybrid mass spectrometry (MS). Here, we have described the techniques used to determine age associated proteomic changes occurring in heads and thoraces across three age groups; 1, 9 and 17 d old A. gambiae and 4 age groups; 1, 9, 17 and 34 d old A. stephensi. We have provided normalised spot volume raw data for all protein spots that were visible on 2D-DIGE images for both species and processed Orbitrap mass spectrometry data. For public access, mass spectrometry raw data are available via ProteomeXchange with identifier PXD002153. A detailed description of this study has been described elsewhere [1].

Original language	English
Pages (from-to)	461-467
Number of pages	7
Journal	Data in Brief
Volume	4
DOIs	https://doi.org/10.1016/j.dib.2015.07.007
Publication status	Published - 1 Sep 2015
Externally published	Yes

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10.1016/j.dib.2015.07.007

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@article{f7eeddc1b1354064a2de8b705cd28768,

title = "Mass spectrometry identification of age-associated proteins from the malaria mosquitoes Anopheles gambiae s.s. and Anopheles stephensi",

abstract = "This study investigated proteomic changes occurring in Anopheles gambiae and Anopheles stephensi during adult mosquito aging. These changes were evaluated using two-dimensional difference gel electrophoresis (2D-DIGE) and the identities of aging related proteins were determined using capillary high-pressure liquid chromatography (capHPLC) coupled with a linear ion-trap (LTQ)-Orbitrap XL hybrid mass spectrometry (MS). Here, we have described the techniques used to determine age associated proteomic changes occurring in heads and thoraces across three age groups; 1, 9 and 17 d old A. gambiae and 4 age groups; 1, 9, 17 and 34 d old A. stephensi. We have provided normalised spot volume raw data for all protein spots that were visible on 2D-DIGE images for both species and processed Orbitrap mass spectrometry data. For public access, mass spectrometry raw data are available via ProteomeXchange with identifier PXD002153. A detailed description of this study has been described elsewhere [1].",

author = "Sikulu, \{Maggy T.\} and James Monkman and Dave, \{Keyur A.\} and Hastie, \{Marcus L.\} and Dale, \{Patricia E.\} and Kitching, \{Roger L.\} and Killeen, \{Gerry F.\} and Kay, \{Brian H.\} and Gorman, \{Jeffry J.\} and Hugo, \{Leon E.\}",

note = "Publisher Copyright: {\textcopyright} 2015 The Authors.",

year = "2015",

month = sep,

day = "1",

doi = "10.1016/j.dib.2015.07.007",

language = "English",

volume = "4",

pages = "461--467",

journal = "Data in Brief",

issn = "2352-3409",

publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Mass spectrometry identification of age-associated proteins from the malaria mosquitoes Anopheles gambiae s.s. and Anopheles stephensi

AU - Sikulu, Maggy T.

AU - Monkman, James

AU - Dave, Keyur A.

AU - Hastie, Marcus L.

AU - Dale, Patricia E.

AU - Kitching, Roger L.

AU - Killeen, Gerry F.

AU - Kay, Brian H.

AU - Gorman, Jeffry J.

AU - Hugo, Leon E.

PY - 2015/9/1

Y1 - 2015/9/1

N2 - This study investigated proteomic changes occurring in Anopheles gambiae and Anopheles stephensi during adult mosquito aging. These changes were evaluated using two-dimensional difference gel electrophoresis (2D-DIGE) and the identities of aging related proteins were determined using capillary high-pressure liquid chromatography (capHPLC) coupled with a linear ion-trap (LTQ)-Orbitrap XL hybrid mass spectrometry (MS). Here, we have described the techniques used to determine age associated proteomic changes occurring in heads and thoraces across three age groups; 1, 9 and 17 d old A. gambiae and 4 age groups; 1, 9, 17 and 34 d old A. stephensi. We have provided normalised spot volume raw data for all protein spots that were visible on 2D-DIGE images for both species and processed Orbitrap mass spectrometry data. For public access, mass spectrometry raw data are available via ProteomeXchange with identifier PXD002153. A detailed description of this study has been described elsewhere [1].

AB - This study investigated proteomic changes occurring in Anopheles gambiae and Anopheles stephensi during adult mosquito aging. These changes were evaluated using two-dimensional difference gel electrophoresis (2D-DIGE) and the identities of aging related proteins were determined using capillary high-pressure liquid chromatography (capHPLC) coupled with a linear ion-trap (LTQ)-Orbitrap XL hybrid mass spectrometry (MS). Here, we have described the techniques used to determine age associated proteomic changes occurring in heads and thoraces across three age groups; 1, 9 and 17 d old A. gambiae and 4 age groups; 1, 9, 17 and 34 d old A. stephensi. We have provided normalised spot volume raw data for all protein spots that were visible on 2D-DIGE images for both species and processed Orbitrap mass spectrometry data. For public access, mass spectrometry raw data are available via ProteomeXchange with identifier PXD002153. A detailed description of this study has been described elsewhere [1].

UR - https://www.scopus.com/pages/publications/84938278283

U2 - 10.1016/j.dib.2015.07.007

DO - 10.1016/j.dib.2015.07.007

M3 - Article

AN - SCOPUS:84938278283

SN - 2352-3409

VL - 4

SP - 461

EP - 467

JO - Data in Brief

JF - Data in Brief

ER -

Mass spectrometry identification of age-associated proteins from the malaria mosquitoes Anopheles gambiae s.s. and Anopheles stephensi

Abstract

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