Mediated microbial biosensor using a novel yeast strain for wastewater BOD measurement

S. P. Trosok; B. T. Driscoll; J. H.T. Luong

doi:10.1007/s002530100674

Mediated microbial biosensor using a novel yeast strain for wastewater BOD measurement

S. P. Trosok
, B. T. Driscoll
, J. H.T. Luong

Research output: Contribution to journal › Article › peer-review

Abstract

Two new yeast strains (SPT1 and SPT2) were isolated and immobilized on glassy carbon electrodes to form microbial biosensors for estimation of biochemical oxygen demand (BOD). Ferricyanide was proven to be the most efficient mediator to shuttle electrons from the redox center of reduced microbial enzymes to the electrode in the presence of excess glucose/glutamic acid (GGA). With a 3-fold greater metabolic assimilation capability and greater responses to various effluent samples, SPT1 was selected for sensor-BOD measurements. BOD estimations for the GGA standard resulted in an extended linear range: 2-100 mg/l. Response reproducibility was ±10% for a GGA standard containing 10 mg BOD/l. For analysis of pulp mill effluents, the BOD detection limit was 2 mg/l with a response time of 5 min.

Original language	English
Pages (from-to)	550-554
Number of pages	5
Journal	Applied Microbiology and Biotechnology
Volume	56
Issue number	3-4
DOIs	https://doi.org/10.1007/s002530100674
Publication status	Published - 2001
Externally published	Yes

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10.1007/s002530100674

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title = "Mediated microbial biosensor using a novel yeast strain for wastewater BOD measurement",

abstract = "Two new yeast strains (SPT1 and SPT2) were isolated and immobilized on glassy carbon electrodes to form microbial biosensors for estimation of biochemical oxygen demand (BOD). Ferricyanide was proven to be the most efficient mediator to shuttle electrons from the redox center of reduced microbial enzymes to the electrode in the presence of excess glucose/glutamic acid (GGA). With a 3-fold greater metabolic assimilation capability and greater responses to various effluent samples, SPT1 was selected for sensor-BOD measurements. BOD estimations for the GGA standard resulted in an extended linear range: 2-100 mg/l. Response reproducibility was ±10\% for a GGA standard containing 10 mg BOD/l. For analysis of pulp mill effluents, the BOD detection limit was 2 mg/l with a response time of 5 min.",

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AU - Trosok, S. P.

AU - Driscoll, B. T.

AU - Luong, J. H.T.

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Y1 - 2001

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AB - Two new yeast strains (SPT1 and SPT2) were isolated and immobilized on glassy carbon electrodes to form microbial biosensors for estimation of biochemical oxygen demand (BOD). Ferricyanide was proven to be the most efficient mediator to shuttle electrons from the redox center of reduced microbial enzymes to the electrode in the presence of excess glucose/glutamic acid (GGA). With a 3-fold greater metabolic assimilation capability and greater responses to various effluent samples, SPT1 was selected for sensor-BOD measurements. BOD estimations for the GGA standard resulted in an extended linear range: 2-100 mg/l. Response reproducibility was ±10% for a GGA standard containing 10 mg BOD/l. For analysis of pulp mill effluents, the BOD detection limit was 2 mg/l with a response time of 5 min.

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JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

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ER -

Mediated microbial biosensor using a novel yeast strain for wastewater BOD measurement

Abstract

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