TY - JOUR
T1 - Micellar electrokinetic capillary chromatography and data alignment analysis
T2 - A new tool in urine profiling
AU - Guillo, Christelle
AU - Barlow, David
AU - Perrett, David
AU - Hanna-Brown, Melissa
PY - 2004/2/20
Y1 - 2004/2/20
N2 - The complex nature of biofluids demands efficient, sensitive and high-resolution analytical methodologies to examine how the 'metabolic fingerprint' changes during disease. This paper describes how sulphated β-cyclodextrin-modified micellar electrokinetic capillary chromatography (SβCD-MECC) has been combined with data alignment analysis and may prove a useful new tool in urine profiling, allowing for separation of over 80 urinary analytes in under 25min. The optimised and validated SβCD-MECC methodology combined with data alignment analysis provides rapid identification of 'mismatches' between urine profiles which are not easily detected with the naked eye as well as a 'similarity score' which indicates the total sum of differences between one profile and another. The combination of SβCD-MECC with data alignment software should prove a useful alternative tool in metabonomic studies for rapid comparison of urine profiles.
AB - The complex nature of biofluids demands efficient, sensitive and high-resolution analytical methodologies to examine how the 'metabolic fingerprint' changes during disease. This paper describes how sulphated β-cyclodextrin-modified micellar electrokinetic capillary chromatography (SβCD-MECC) has been combined with data alignment analysis and may prove a useful new tool in urine profiling, allowing for separation of over 80 urinary analytes in under 25min. The optimised and validated SβCD-MECC methodology combined with data alignment analysis provides rapid identification of 'mismatches' between urine profiles which are not easily detected with the naked eye as well as a 'similarity score' which indicates the total sum of differences between one profile and another. The combination of SβCD-MECC with data alignment software should prove a useful alternative tool in metabonomic studies for rapid comparison of urine profiles.
KW - Cyclodextrins
KW - Data alignment
KW - Electrokinetic chromatography
UR - https://www.scopus.com/pages/publications/0346023063
U2 - 10.1016/j.chroma.2003.11.102
DO - 10.1016/j.chroma.2003.11.102
M3 - Article
C2 - 14971504
AN - SCOPUS:0346023063
SN - 0021-9673
VL - 1027
SP - 203
EP - 212
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1-2
ER -