Molecular dissection of a bifidobacterial replicon

Michelle Cronin; Moritz Knobel; Mary O'Connell-Motherway; Gerald F. Fitzgerald; Douwe Van Sinderen

doi:10.1128/AEM.01630-07

Molecular dissection of a bifidobacterial replicon

Michelle Cronin
, Moritz Knobel
, Mary O'Connell-Motherway
, Gerald F. Fitzgerald
, Douwe Van Sinderen

University College Cork

Research output: Contribution to journal › Article › peer-review

Abstract

The 2.1-kb cryptic plasmid pCIBAO89 from Bifidobacterium asteroides harbors a 1.4-kb segment which is sufficient for its autonomous replication. The segment is divided into two parts, the presumed replication origin, ori89, and the rep gene encoding the putative 41-kDa Rep89 replication initiation protein. This minimal replication region of pCIBAO89 was functionally dissected by transcriptional analyses as well as by DNA-binding studies, and the information obtained was exploited to create a number of Escherichia coli-Bifidobacterium shuttle vectors capable of transforming various bifidobacteria with an efficiency of up to 10⁶ transformants/μg DNA.

Original language	English
Pages (from-to)	7858-7866
Number of pages	9
Journal	Applied and Environmental Microbiology
Volume	73
Issue number	24
DOIs	https://doi.org/10.1128/AEM.01630-07
Publication status	Published - Dec 2007

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AU - O'Connell-Motherway, Mary

AU - Fitzgerald, Gerald F.

AU - Van Sinderen, Douwe

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UR - https://www.scopus.com/pages/publications/37349034983

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JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

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ER -

Molecular dissection of a bifidobacterial replicon

Abstract

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