Molecular genetic study of species and strain variability in bifi dobacteria population in intestinal microfl ora of breast-fed infants and their mothers

E. V. Kulagina; A. N. Shkoporov; L. I. Kafarskaia; E. V. Khokhlova; N. N. Volodin; E. E. Donskikh; O. V. Korshunova; B. A. Efimov

doi:10.1007/s10517-010-1069-y

Molecular genetic study of species and strain variability in bifi dobacteria population in intestinal microfl ora of breast-fed infants and their mothers

E. V. Kulagina
, A. N. Shkoporov
, L. I. Kafarskaia
, E. V. Khokhlova
, N. N. Volodin
, E. E. Donskikh
, O. V. Korshunova
, B. A. Efimov

Pirogov Russian National Research Medical University

Research output: Contribution to journal › Article › peer-review

Abstract

Qualitative and quantitative composition of enteric bifi dofl ora was studied in a group of 13 mother-infant pairs. Pure cultures of Bifi dobacterium strains were isolated from feces and their species were identifi ed by PCR with species-specifi c primers or by partial sequencing of 16S rDNA. The strains were compared by REP-PCR. The most incident Bifi dobacterium species in mothers were B. longum and B. adolescentis. The infants were mainly colonized by B. bifi dum and B. longum. The mother and her baby were colonized by the same Bifi dobacterium species in 9 of 13 cases. In 5 (38.5%) of these cases, these pairs of strains were identical by their REP-PCR profi les. These strains belonged to B. longum in one case, B. bifi dum in 3 cases, and B. adolescentis in 1 case. Our results support the hypothesis on early colonization of infants with maternal bifi dobacterium strains.

Original language	English
Pages (from-to)	61-64
Number of pages	4
Journal	Bulletin of Experimental Biology and Medicine
Volume	150
Issue number	1
DOIs	https://doi.org/10.1007/s10517-010-1069-y
Publication status	Published - Dec 2010
Externally published	Yes

Keywords

Bifi dobacteria
Enteric microfl ora
REP-PCR

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10.1007/s10517-010-1069-y

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Kulagina, E. V., Shkoporov, A. N., Kafarskaia, L. I., Khokhlova, E. V., Volodin, N. N., Donskikh, E. E., Korshunova, O. V., & Efimov, B. A. (2010). Molecular genetic study of species and strain variability in bifi dobacteria population in intestinal microfl ora of breast-fed infants and their mothers. Bulletin of Experimental Biology and Medicine, 150(1), 61-64. https://doi.org/10.1007/s10517-010-1069-y

@article{12ccec44fbae46bebdcd45649fa09bd9,

title = "Molecular genetic study of species and strain variability in bifi dobacteria population in intestinal microfl ora of breast-fed infants and their mothers",

abstract = "Qualitative and quantitative composition of enteric bifi dofl ora was studied in a group of 13 mother-infant pairs. Pure cultures of Bifi dobacterium strains were isolated from feces and their species were identifi ed by PCR with species-specifi c primers or by partial sequencing of 16S rDNA. The strains were compared by REP-PCR. The most incident Bifi dobacterium species in mothers were B. longum and B. adolescentis. The infants were mainly colonized by B. bifi dum and B. longum. The mother and her baby were colonized by the same Bifi dobacterium species in 9 of 13 cases. In 5 (38.5\%) of these cases, these pairs of strains were identical by their REP-PCR profi les. These strains belonged to B. longum in one case, B. bifi dum in 3 cases, and B. adolescentis in 1 case. Our results support the hypothesis on early colonization of infants with maternal bifi dobacterium strains.",

keywords = "Bifi dobacteria, Enteric microfl ora, REP-PCR",

author = "Kulagina, \{E. V.\} and Shkoporov, \{A. N.\} and Kafarskaia, \{L. I.\} and Khokhlova, \{E. V.\} and Volodin, \{N. N.\} and Donskikh, \{E. E.\} and Korshunova, \{O. V.\} and Efimov, \{B. A.\}",

year = "2010",

month = dec,

doi = "10.1007/s10517-010-1069-y",

language = "English",

volume = "150",

pages = "61--64",

journal = "Bulletin of Experimental Biology and Medicine",

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number = "1",

}

Kulagina, EV, Shkoporov, AN, Kafarskaia, LI, Khokhlova, EV, Volodin, NN, Donskikh, EE, Korshunova, OV & Efimov, BA 2010, 'Molecular genetic study of species and strain variability in bifi dobacteria population in intestinal microfl ora of breast-fed infants and their mothers', Bulletin of Experimental Biology and Medicine, vol. 150, no. 1, pp. 61-64. https://doi.org/10.1007/s10517-010-1069-y

Molecular genetic study of species and strain variability in bifi dobacteria population in intestinal microfl ora of breast-fed infants and their mothers. / Kulagina, E. V.; Shkoporov, A. N.; Kafarskaia, L. I. et al.
In: Bulletin of Experimental Biology and Medicine, Vol. 150, No. 1, 12.2010, p. 61-64.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Molecular genetic study of species and strain variability in bifi dobacteria population in intestinal microfl ora of breast-fed infants and their mothers

AU - Kulagina, E. V.

AU - Shkoporov, A. N.

AU - Kafarskaia, L. I.

AU - Khokhlova, E. V.

AU - Volodin, N. N.

AU - Donskikh, E. E.

AU - Korshunova, O. V.

AU - Efimov, B. A.

PY - 2010/12

Y1 - 2010/12

N2 - Qualitative and quantitative composition of enteric bifi dofl ora was studied in a group of 13 mother-infant pairs. Pure cultures of Bifi dobacterium strains were isolated from feces and their species were identifi ed by PCR with species-specifi c primers or by partial sequencing of 16S rDNA. The strains were compared by REP-PCR. The most incident Bifi dobacterium species in mothers were B. longum and B. adolescentis. The infants were mainly colonized by B. bifi dum and B. longum. The mother and her baby were colonized by the same Bifi dobacterium species in 9 of 13 cases. In 5 (38.5%) of these cases, these pairs of strains were identical by their REP-PCR profi les. These strains belonged to B. longum in one case, B. bifi dum in 3 cases, and B. adolescentis in 1 case. Our results support the hypothesis on early colonization of infants with maternal bifi dobacterium strains.

AB - Qualitative and quantitative composition of enteric bifi dofl ora was studied in a group of 13 mother-infant pairs. Pure cultures of Bifi dobacterium strains were isolated from feces and their species were identifi ed by PCR with species-specifi c primers or by partial sequencing of 16S rDNA. The strains were compared by REP-PCR. The most incident Bifi dobacterium species in mothers were B. longum and B. adolescentis. The infants were mainly colonized by B. bifi dum and B. longum. The mother and her baby were colonized by the same Bifi dobacterium species in 9 of 13 cases. In 5 (38.5%) of these cases, these pairs of strains were identical by their REP-PCR profi les. These strains belonged to B. longum in one case, B. bifi dum in 3 cases, and B. adolescentis in 1 case. Our results support the hypothesis on early colonization of infants with maternal bifi dobacterium strains.

KW - Bifi dobacteria

KW - Enteric microfl ora

KW - REP-PCR

UR - https://www.scopus.com/pages/publications/79951643560

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DO - 10.1007/s10517-010-1069-y

M3 - Article

C2 - 21161053

AN - SCOPUS:79951643560

SN - 0007-4888

VL - 150

SP - 61

EP - 64

JO - Bulletin of Experimental Biology and Medicine

JF - Bulletin of Experimental Biology and Medicine

IS - 1

ER -

Molecular genetic study of species and strain variability in bifi dobacteria population in intestinal microfl ora of breast-fed infants and their mothers

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