TY - JOUR
T1 - Multi-Parametric o2 imaging in three-dimensional neural cell models with the phosphorescent probes
AU - Dmitriev, Ruslan I.
AU - Papkovsky, Dmitri B.
N1 - Publisher Copyright:
© Springer Science+Business Media New York 2015.
PY - 2015
Y1 - 2015
N2 - Recent progress in bio- imaging has allowed detailed mechanistic studies of neural cell function in complex 3D tissue models including multi cellular aggregates, neurospheres, excised brain slices, ganglia, and organoids. Molecular oxygen (O2) is an important metabolite and an environmental parameter which determines the viability and physiological status of neural cells within tissue. Here we describe standard method for monitoring O2 in 3D tissue models using phosphorescence lifetime imaging microscopy (PLIM) and cell-penetrating O2 -sensing probes. The O2 probes can be multiplexed with many conventional fl uorescence based live cell biomarkers and also end-point immunofl uorescence staining. The multi-parametric O2 imaging method is particularly useful for areas such as stem cell development and differentiation, hypoxia research, neurodegenerative disorders, regeneration of brain tissue, evaluation of new drugs, and development of novel tissue models.
AB - Recent progress in bio- imaging has allowed detailed mechanistic studies of neural cell function in complex 3D tissue models including multi cellular aggregates, neurospheres, excised brain slices, ganglia, and organoids. Molecular oxygen (O2) is an important metabolite and an environmental parameter which determines the viability and physiological status of neural cells within tissue. Here we describe standard method for monitoring O2 in 3D tissue models using phosphorescence lifetime imaging microscopy (PLIM) and cell-penetrating O2 -sensing probes. The O2 probes can be multiplexed with many conventional fl uorescence based live cell biomarkers and also end-point immunofl uorescence staining. The multi-parametric O2 imaging method is particularly useful for areas such as stem cell development and differentiation, hypoxia research, neurodegenerative disorders, regeneration of brain tissue, evaluation of new drugs, and development of novel tissue models.
KW - High- resolution microscopy
KW - Imaging
KW - Oxygen
KW - Phosphorescence quenching
KW - PLIM
UR - https://www.scopus.com/pages/publications/84954597813
U2 - 10.1007/978-1-4939-2152-2_5
DO - 10.1007/978-1-4939-2152-2_5
M3 - Article
C2 - 25431057
AN - SCOPUS:84954597813
SN - 1064-3745
VL - 1254
SP - 55
EP - 71
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -