Multi-Parametric o2 imaging in three-dimensional neural cell models with the phosphorescent probes

Ruslan I. Dmitriev; Dmitri B. Papkovsky

doi:10.1007/978-1-4939-2152-2_5

Multi-Parametric o₂ imaging in three-dimensional neural cell models with the phosphorescent probes

Ruslan I. Dmitriev
, Dmitri B. Papkovsky

School of Biochemistry and Cell Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Recent progress in bio- imaging has allowed detailed mechanistic studies of neural cell function in complex 3D tissue models including multi cellular aggregates, neurospheres, excised brain slices, ganglia, and organoids. Molecular oxygen (O₂) is an important metabolite and an environmental parameter which determines the viability and physiological status of neural cells within tissue. Here we describe standard method for monitoring O₂ in 3D tissue models using phosphorescence lifetime imaging microscopy (PLIM) and cell-penetrating O₂ -sensing probes. The O₂ probes can be multiplexed with many conventional fl uorescence based live cell biomarkers and also end-point immunofl uorescence staining. The multi-parametric O₂ imaging method is particularly useful for areas such as stem cell development and differentiation, hypoxia research, neurodegenerative disorders, regeneration of brain tissue, evaluation of new drugs, and development of novel tissue models.

Original language	English
Pages (from-to)	55-71
Number of pages	17
Journal	Methods in Molecular Biology
Volume	1254
DOIs	https://doi.org/10.1007/978-1-4939-2152-2_5
Publication status	Published - 2015

Keywords

High- resolution microscopy
Imaging
Oxygen
Phosphorescence quenching
PLIM

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10.1007/978-1-4939-2152-2_5

Cite this

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title = "Multi-Parametric o2 imaging in three-dimensional neural cell models with the phosphorescent probes",

abstract = "Recent progress in bio- imaging has allowed detailed mechanistic studies of neural cell function in complex 3D tissue models including multi cellular aggregates, neurospheres, excised brain slices, ganglia, and organoids. Molecular oxygen (O2) is an important metabolite and an environmental parameter which determines the viability and physiological status of neural cells within tissue. Here we describe standard method for monitoring O2 in 3D tissue models using phosphorescence lifetime imaging microscopy (PLIM) and cell-penetrating O2 -sensing probes. The O2 probes can be multiplexed with many conventional fl uorescence based live cell biomarkers and also end-point immunofl uorescence staining. The multi-parametric O2 imaging method is particularly useful for areas such as stem cell development and differentiation, hypoxia research, neurodegenerative disorders, regeneration of brain tissue, evaluation of new drugs, and development of novel tissue models.",

keywords = "High- resolution microscopy, Imaging, Oxygen, Phosphorescence quenching, PLIM",

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year = "2015",

doi = "10.1007/978-1-4939-2152-2\_5",

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T1 - Multi-Parametric o2 imaging in three-dimensional neural cell models with the phosphorescent probes

AU - Dmitriev, Ruslan I.

AU - Papkovsky, Dmitri B.

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PY - 2015

Y1 - 2015

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AB - Recent progress in bio- imaging has allowed detailed mechanistic studies of neural cell function in complex 3D tissue models including multi cellular aggregates, neurospheres, excised brain slices, ganglia, and organoids. Molecular oxygen (O2) is an important metabolite and an environmental parameter which determines the viability and physiological status of neural cells within tissue. Here we describe standard method for monitoring O2 in 3D tissue models using phosphorescence lifetime imaging microscopy (PLIM) and cell-penetrating O2 -sensing probes. The O2 probes can be multiplexed with many conventional fl uorescence based live cell biomarkers and also end-point immunofl uorescence staining. The multi-parametric O2 imaging method is particularly useful for areas such as stem cell development and differentiation, hypoxia research, neurodegenerative disorders, regeneration of brain tissue, evaluation of new drugs, and development of novel tissue models.

KW - High- resolution microscopy

KW - Imaging

KW - Oxygen

KW - Phosphorescence quenching

KW - PLIM

UR - https://www.scopus.com/pages/publications/84954597813

U2 - 10.1007/978-1-4939-2152-2_5

DO - 10.1007/978-1-4939-2152-2_5

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AN - SCOPUS:84954597813

SN - 1064-3745

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JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Multi-Parametric o₂ imaging in three-dimensional neural cell models with the phosphorescent probes

Abstract

Keywords

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