Abstract
Cleavage of 16S ribosomal RNA (rRNA) from E. coli "hammerhead" type ribozymes as well as by RNAase iI in the presence of "hymeric" (2'-deoxy-F-thymidine containing) oligonucleotides has been studied. The conditions for the cleavage of a desired single internucleotide bond have been found for a large molecule with a very complicated secondary and three-dimensional structure.
| Translated title of the contribution | Directed cleavage of the 16S rRNA molecule at a single internucleotide bond |
|---|---|
| Original language | Russian |
| Pages (from-to) | 297-307 |
| Number of pages | 11 |
| Journal | Biokhimiya |
| Volume | 60 |
| Issue number | 2 |
| Publication status | Published - Feb 1995 |