Next-generation sequencing technologies and their impact on microbial genomics

Next-generation sequencing technologies have had a dramatic impact in the field of genomic research through the provision of a low cost, high-throughput alternative to traditional capillary sequencers.These new sequencingmethods have surpassed their original scope and now provide a range of utility-based applications, which allow for a more comprehensive analysis of the structure and content of microbial genomes than was previously possible.With the commercialization of a third generation of sequencing technologiesimminent, we discuss the applications of current next-generation sequencingmethods and explore their impact on and contribution to microbial genome research.