Non-uniform Fourier transform based rotational alignment in single-particle Cryo-EM

Research output: Chapter in Book/Report/Conference proceedingsChapterpeer-review

Abstract

In the realm of single-particle Cryo-EM's 2D align-ment problem, conventional practice necessitates interpolation as an intermediate step before applying the Fourier transform to address the in-plane rotation angle. Unfortunately, this interpo-lation process tends to introduce errors, making the process less precise. In contrast, the Non-uniform Fourier transform offers a more direct and accurate mathematical foundation for analyzing and computing the in-plane rotation angle, eliminating the need for interpolation in related problem-solving tasks. Leveraging the capabilities of the Non-uniform Fourier transform, we have developed an algorithm for in-plane rotation angle estimation. To underscore its potential and applicability in the field of single-particle Cryo-EM, we have conducted a direct comparison with the traditional Fourier transform, demonstrating the superior performance of the Non-uniform Fourier transform.

Original languageEnglish
Title of host publicationIEEE ITAIC 2023 - IEEE 11th Joint International Information Technology and Artificial Intelligence Conference
EditorsBing Xu, Kefen Mou
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1457-1463
Number of pages7
ISBN (Electronic)9798350333664
DOIs
Publication statusPublished - 2023
Event11th Joint International Information Technology and Artificial Intelligence Conference, ITAIC 2023 - Chongqing, China
Duration: 8 Dec 202310 Dec 2023

Publication series

NameIEEE Joint International Information Technology and Artificial Intelligence Conference (ITAIC)
ISSN (Print)2693-2865

Conference

Conference11th Joint International Information Technology and Artificial Intelligence Conference, ITAIC 2023
Country/TerritoryChina
CityChongqing
Period8/12/2310/12/23

Keywords

  • 2D alignment
  • in-plane rotation
  • Non-uniform Fourier transform
  • single- particle Cryo- Em

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