TY - JOUR
T1 - Novel Escherichia coli-Infecting Bacteriophages Isolated from Uganda That Target Human Clinical Isolates
AU - Nale, Janet Yakubu
AU - Chan, Benjamin
AU - Nnadi, Nnaemeka Emmanuel
AU - Cheng, Jeffrey Kwok Jone
AU - Matts, Susan
AU - Nezam-Abadi, Neda
AU - Turkington, Christopher Jason Richard
AU - Charreton, Lucie Manon
AU - Bola, Harroop
AU - Nazir, Ramez
AU - Hoza, Abubakar Shaaban
AU - Wamala, Samuel Posian
AU - Ibanda, Ivan
AU - Maina, Alice Nyambura
AU - Apopo, Auleria Ajiambo
AU - Msoffe, Venance Theophil
AU - Moremi, Nyambura
AU - Moore, Grace Wanjiru
AU - Asiimwe, Ismail
AU - Namatovu, Alice
AU - Mutumba, Paul
AU - Kamya, Deus
AU - Nabunje, Ritah
AU - Nakabugo, Immaculate
AU - Kazwala, Rudovick Ruben
AU - Kangethe, Erastus
AU - Negash, Abel Abera
AU - Watelo, Arthur Kalyebi
AU - Bukamba, Nelson
AU - Muhindo, Gideon
AU - Lubowa, Nathan Musisi
AU - Jillani, Ngalla
AU - Nyachieo, Atunga
AU - Nasinyama, George
AU - Nakavuma, Jesca
AU - Millard, Andrew
AU - Nagel, Tobi Elaine
AU - Clokie, Martha Rebecca Jane
N1 - Publisher Copyright:
© Copyright 2023, Mary Ann Liebert, Inc., publishers 2023.
PY - 2023/9/1
Y1 - 2023/9/1
N2 - Background: The antimicrobial resistance catastrophe is a growing global health threat and predicted to be worse in developing countries. Phages for Global Health (PGH) is training scientists in these regions to isolate relevant therapeutic phages for pathogenic bacteria within their locality, and thus contributing to making phage technology universally available. Materials and Methods: During the inaugural PGH workshop in East Africa, samples from Ugandan municipal sewage facilities were collected and two novel Escherichia coli lytic phages were isolated and characterized. Results: The phages, UP19 (capsid diameter ∼100 nm, contractile tail ∼120/20 nm) and UP30 (capsid diameter ∼70 nm, noncontractile tail of ∼170/20 nm), lysed ∼82% and ∼36% of the 11 clinical isolates examined, respectively. The genomes of UP19 (171.402 kb, 282 CDS) and UP30 (49.834 kb, 75 CDS) closely match the genera Dhakavirus and Tunavirus, respectively. Conclusion: The phages isolated have therapeutic potential for further development against E. coli infections.
AB - Background: The antimicrobial resistance catastrophe is a growing global health threat and predicted to be worse in developing countries. Phages for Global Health (PGH) is training scientists in these regions to isolate relevant therapeutic phages for pathogenic bacteria within their locality, and thus contributing to making phage technology universally available. Materials and Methods: During the inaugural PGH workshop in East Africa, samples from Ugandan municipal sewage facilities were collected and two novel Escherichia coli lytic phages were isolated and characterized. Results: The phages, UP19 (capsid diameter ∼100 nm, contractile tail ∼120/20 nm) and UP30 (capsid diameter ∼70 nm, noncontractile tail of ∼170/20 nm), lysed ∼82% and ∼36% of the 11 clinical isolates examined, respectively. The genomes of UP19 (171.402 kb, 282 CDS) and UP30 (49.834 kb, 75 CDS) closely match the genera Dhakavirus and Tunavirus, respectively. Conclusion: The phages isolated have therapeutic potential for further development against E. coli infections.
KW - antimicrobial resistance
KW - Escherichia coli phage
KW - phage therapy
KW - Phages for Global Health
UR - https://www.scopus.com/pages/publications/85173460007
U2 - 10.1089/phage.2023.0012
DO - 10.1089/phage.2023.0012
M3 - Review article
AN - SCOPUS:85173460007
SN - 2641-6530
VL - 4
SP - 141
EP - 149
JO - PHAGE: Therapy, Applications, and Research
JF - PHAGE: Therapy, Applications, and Research
IS - 3
ER -
SDG 11 Sustainable Cities and Communities