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One-step antibody immobilization-based high sensitivity immunoassay procedure for potential in vitro diagnostics

  • Sandeep Kumar Vashist
  • , Felix von Stetten
  • , Roland Zengerle
  • , John H.T. Luong

Research output: Chapter in Book/Report/Conference proceedingsConference proceedingpeer-review

Abstract

A potential one-step antibody (Ab) immobilization strategy was developed for the leach-proof binding of capture Ab to the polystyrene microtiter plate (MTP) [1-3]. It enables the development of high sensitivity, cost-effective and analytically superior immunoassay (IA) for potential in vitro diagnostic (IVD) kits. The NIF-based IA detected HFA in the range of 4.9 pg mL-1-20 ng mL-1 with limit of detection (LOD) of 7 pg mL-1 (Fig. 1B (a)). The analytical sensitivity was 10 pg mL-1, which was 51-fold more sensitive than the convention immobilization format (CIF) based commercial ELISA.

Original languageEnglish
Title of host publicationMikroSystemTechnik Kongress 2015 "MEMS, Mikroelektronik, Systeme", Proceedings
PublisherVDE Verlag GmbH
Pages501-503
Number of pages3
ISBN (Electronic)9783800741007
Publication statusPublished - 2015
EventMikroSystemTechnik Kongress 2015: MEMS, Mikroelektronik, Systeme - MikroSystemTechnik Conference 2015: MEMS, Microelectronics, Systems - Karlsruhe, Germany
Duration: 26 Oct 201528 Oct 2015

Publication series

NameMikroSystemTechnik Kongress 2015 "MEMS, Mikroelektronik, Systeme", Proceedings

Conference

ConferenceMikroSystemTechnik Kongress 2015: MEMS, Mikroelektronik, Systeme - MikroSystemTechnik Conference 2015: MEMS, Microelectronics, Systems
Country/TerritoryGermany
CityKarlsruhe
Period26/10/1528/10/15

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