TY - GEN
T1 - Phosphorescence based oxygen sensors and probes for biomedical research
AU - Papkovsky, Dmitri B.
AU - Zhdanov, Alexander V.
N1 - Publisher Copyright:
© 2017 SPIE.
PY - 2017
Y1 - 2017
N2 - A variety of in vitro and ex-vivo cell and tissue models are being used in biomedical research, but for many of them control of the cellular microenvironment, particularly oxygenation state and intracellular O2 levels, is inadequate. Since O2 is a key parameter and biomarker of cellular function, implementation of reliable in situ control and knowledge of actual O2 levels in different compartments of biological samples is of critical importance. The versatile and flexible technology of O2 sensing and imaging based on phosphorescence quenching provides such capabilities. In recent years, various O2 sensing systems, which operate with solid-state sensors, soluble probes or imaging (nano)sensors in conjunction with portable handheld instruments, commercial plate readers or live cell imaging platforms, have been developed, which are suitable for routine use in many research labs to perform a range of important analytical and biomedical tasks. Here we overview the available O2 sensing solutions, their analytical features, and describe how they can be integrated in the current paradigm of biomedical research. Representative examples of the use of such systems in complex physiological studies with advanced tissue and disease models are given, in which they provide strict environmental control of dissolved and gaseous O2 (macroscopically and microscopically, by point measurements and high-resolution imaging in 2D and 3D), and important information about cellular function and changes in tissue metabolism under different conditions and treatments.
AB - A variety of in vitro and ex-vivo cell and tissue models are being used in biomedical research, but for many of them control of the cellular microenvironment, particularly oxygenation state and intracellular O2 levels, is inadequate. Since O2 is a key parameter and biomarker of cellular function, implementation of reliable in situ control and knowledge of actual O2 levels in different compartments of biological samples is of critical importance. The versatile and flexible technology of O2 sensing and imaging based on phosphorescence quenching provides such capabilities. In recent years, various O2 sensing systems, which operate with solid-state sensors, soluble probes or imaging (nano)sensors in conjunction with portable handheld instruments, commercial plate readers or live cell imaging platforms, have been developed, which are suitable for routine use in many research labs to perform a range of important analytical and biomedical tasks. Here we overview the available O2 sensing solutions, their analytical features, and describe how they can be integrated in the current paradigm of biomedical research. Representative examples of the use of such systems in complex physiological studies with advanced tissue and disease models are given, in which they provide strict environmental control of dissolved and gaseous O2 (macroscopically and microscopically, by point measurements and high-resolution imaging in 2D and 3D), and important information about cellular function and changes in tissue metabolism under different conditions and treatments.
KW - cell and tissue oxygenation
KW - cell-permeable phosphorescent probes
KW - Optical oxygen sensing and imaging
KW - phosphorescence quenching method
KW - Pt-porphyrins
KW - solid-sate sensors
UR - https://www.scopus.com/pages/publications/85028966213
U2 - 10.1117/12.2261960
DO - 10.1117/12.2261960
M3 - Conference proceeding
AN - SCOPUS:85028966213
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Advanced Environmental, Chemical, and Biological Sensing Technologies XIV
A2 - Vo-Dinh, Tuan
A2 - Lieberman, Robert A.
PB - SPIE
T2 - Advanced Environmental, Chemical, and Biological Sensing Technologies XIV 2017
Y2 - 9 April 2017 through 10 April 2017
ER -