Picomolar detection of protease using peptide/single walled carbon nanotube/gold nanoparticle-modified electrode

Khaled A. Mahmoud; Sabahudin Hrapovic; John H.T. Luong

doi:10.1021/nn8000774

Picomolar detection of protease using peptide/single walled carbon nanotube/gold nanoparticle-modified electrode

Khaled A. Mahmoud
, Sabahudin Hrapovic
, John H.T. Luong

School of Chemistry

National Research Council of Canada

Research output: Contribution to journal › Article › peer-review

Abstract

Picomolar electrochemical detection of human immunodeficiency virus type-1 protease (HIV-1 PR) using ferrocene (Fc)-pepstatin-modified surfaces has been presented. Gold electrode surface was modified with gold nanoparticles (AuNP) or thiolated single walled carbon nanotubes/ gold nanoparticles (SWCNT/AuNP). Thiol-terminated Fc-pepstatin was then self-assembled on such surfaces as confirmed by Raman spectroscopy and scanning electron microscope. The interaction between the Fc-pepstatin-modified substrates and HIV-1 PR was studied by cyclic voltammetry and electrochemical impedance spectroscopy. Both electrode materials showed enhanced electrochemical responses to increasing concentrations of HIV-1 PR with shifting to higher potentials as well as decrease in the overall signal intensity. However, the sensing electrode modified with thiolated SWCNTs/AuNPs showed remarkable detection sensitivity with an estimated detection limit of 0.8 pM.

Original language	English
Pages (from-to)	1051-1057
Number of pages	7
Journal	ACS Nano
Volume	2
Issue number	5
DOIs	https://doi.org/10.1021/nn8000774
Publication status	Published - May 2008

Keywords

Biosensor
Carbon nanotubes
Ferrocene-pepstatin inhibitor
Gold nanoparticles
Protease

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/nn8000774

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title = "Picomolar detection of protease using peptide/single walled carbon nanotube/gold nanoparticle-modified electrode",

abstract = "Picomolar electrochemical detection of human immunodeficiency virus type-1 protease (HIV-1 PR) using ferrocene (Fc)-pepstatin-modified surfaces has been presented. Gold electrode surface was modified with gold nanoparticles (AuNP) or thiolated single walled carbon nanotubes/ gold nanoparticles (SWCNT/AuNP). Thiol-terminated Fc-pepstatin was then self-assembled on such surfaces as confirmed by Raman spectroscopy and scanning electron microscope. The interaction between the Fc-pepstatin-modified substrates and HIV-1 PR was studied by cyclic voltammetry and electrochemical impedance spectroscopy. Both electrode materials showed enhanced electrochemical responses to increasing concentrations of HIV-1 PR with shifting to higher potentials as well as decrease in the overall signal intensity. However, the sensing electrode modified with thiolated SWCNTs/AuNPs showed remarkable detection sensitivity with an estimated detection limit of 0.8 pM.",

keywords = "Biosensor, Carbon nanotubes, Ferrocene-pepstatin inhibitor, Gold nanoparticles, Protease",

author = "Mahmoud, \{Khaled A.\} and Sabahudin Hrapovic and Luong, \{John H.T.\}",

year = "2008",

month = may,

doi = "10.1021/nn8000774",

language = "English",

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pages = "1051--1057",

journal = "ACS Nano",

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T1 - Picomolar detection of protease using peptide/single walled carbon nanotube/gold nanoparticle-modified electrode

AU - Mahmoud, Khaled A.

AU - Hrapovic, Sabahudin

AU - Luong, John H.T.

PY - 2008/5

Y1 - 2008/5

N2 - Picomolar electrochemical detection of human immunodeficiency virus type-1 protease (HIV-1 PR) using ferrocene (Fc)-pepstatin-modified surfaces has been presented. Gold electrode surface was modified with gold nanoparticles (AuNP) or thiolated single walled carbon nanotubes/ gold nanoparticles (SWCNT/AuNP). Thiol-terminated Fc-pepstatin was then self-assembled on such surfaces as confirmed by Raman spectroscopy and scanning electron microscope. The interaction between the Fc-pepstatin-modified substrates and HIV-1 PR was studied by cyclic voltammetry and electrochemical impedance spectroscopy. Both electrode materials showed enhanced electrochemical responses to increasing concentrations of HIV-1 PR with shifting to higher potentials as well as decrease in the overall signal intensity. However, the sensing electrode modified with thiolated SWCNTs/AuNPs showed remarkable detection sensitivity with an estimated detection limit of 0.8 pM.

AB - Picomolar electrochemical detection of human immunodeficiency virus type-1 protease (HIV-1 PR) using ferrocene (Fc)-pepstatin-modified surfaces has been presented. Gold electrode surface was modified with gold nanoparticles (AuNP) or thiolated single walled carbon nanotubes/ gold nanoparticles (SWCNT/AuNP). Thiol-terminated Fc-pepstatin was then self-assembled on such surfaces as confirmed by Raman spectroscopy and scanning electron microscope. The interaction between the Fc-pepstatin-modified substrates and HIV-1 PR was studied by cyclic voltammetry and electrochemical impedance spectroscopy. Both electrode materials showed enhanced electrochemical responses to increasing concentrations of HIV-1 PR with shifting to higher potentials as well as decrease in the overall signal intensity. However, the sensing electrode modified with thiolated SWCNTs/AuNPs showed remarkable detection sensitivity with an estimated detection limit of 0.8 pM.

KW - Biosensor

KW - Carbon nanotubes

KW - Ferrocene-pepstatin inhibitor

KW - Gold nanoparticles

KW - Protease

UR - https://www.scopus.com/pages/publications/45849114789

U2 - 10.1021/nn8000774

DO - 10.1021/nn8000774

M3 - Article

C2 - 19206503

AN - SCOPUS:45849114789

SN - 1936-0851

VL - 2

SP - 1051

EP - 1057

JO - ACS Nano

JF - ACS Nano

IS - 5

ER -

Picomolar detection of protease using peptide/single walled carbon nanotube/gold nanoparticle-modified electrode

Abstract

Keywords

UN SDGs

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