Abstract
In this communication, we describe a simple and robust method for the covalent bonding of poly(vinyl alcohol) (PVA) on a silanized poly(dimethylsiloxane) (PDMS) surface. Nonspecific adsorption of proteins via hydrophobic-hydrophobic interactions of the PVA-coated surface is greatly reduced, and biomolecules can be rapidly anchored on the PVA-coated surface with high loading and uniformity. On the basis of a sandwich immunoassay with the anti-rabbit IgG and IgG pair as a model, the detection limit for IgG is down to 1 pg/mL with linearity up to 11 μg/mL, the levels often encountered in biological, forensic, and environmental samples.
| Original language | English |
|---|---|
| Pages (from-to) | 281-284 |
| Number of pages | 4 |
| Journal | Bioconjugate Chemistry |
| Volume | 18 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2007 |
| Externally published | Yes |