Potential of using real-time PCR-based detection of spoilage yeast in fruit juice - A preliminary study

Garrett D. Casey; Alan D.W. Dobson

doi:10.1016/j.ijfoodmicro.2003.09.002

Potential of using real-time PCR-based detection of spoilage yeast in fruit juice - A preliminary study

Garrett D. Casey
, Alan D.W. Dobson

University College Cork

Research output: Contribution to journal › Article › peer-review

Abstract

A real-time PCR system was used to differentiate between the common spoilage yeasts, Zygosaccharomyces bailii, Zygosaccharomyces rouxii, Candida krusei, Rhodotorula glutinis and Saccharomyces cerevisiae, based on melting peak T_m analysis of the 5.8S rDNA subunit and the adjacent ITS2 region of these yeasts. By using the real-time PCR system and by targeting the citrate synthase (cs 1) gene of C. krusei, it was possible to develop a sensitive detection system to both identify and quantitate the level of C. krusei growth in an artificially contaminated apple juice sample.

Original language	English
Pages (from-to)	327-335
Number of pages	9
Journal	International Journal of Food Microbiology
Volume	91
Issue number	3
DOIs	https://doi.org/10.1016/j.ijfoodmicro.2003.09.002
Publication status	Published - 15 Mar 2004

Keywords

Candida krusei
Real-time PCR

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10.1016/j.ijfoodmicro.2003.09.002

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title = "Potential of using real-time PCR-based detection of spoilage yeast in fruit juice - A preliminary study",

abstract = "A real-time PCR system was used to differentiate between the common spoilage yeasts, Zygosaccharomyces bailii, Zygosaccharomyces rouxii, Candida krusei, Rhodotorula glutinis and Saccharomyces cerevisiae, based on melting peak Tm analysis of the 5.8S rDNA subunit and the adjacent ITS2 region of these yeasts. By using the real-time PCR system and by targeting the citrate synthase (cs 1) gene of C. krusei, it was possible to develop a sensitive detection system to both identify and quantitate the level of C. krusei growth in an artificially contaminated apple juice sample.",

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AU - Dobson, Alan D.W.

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N2 - A real-time PCR system was used to differentiate between the common spoilage yeasts, Zygosaccharomyces bailii, Zygosaccharomyces rouxii, Candida krusei, Rhodotorula glutinis and Saccharomyces cerevisiae, based on melting peak Tm analysis of the 5.8S rDNA subunit and the adjacent ITS2 region of these yeasts. By using the real-time PCR system and by targeting the citrate synthase (cs 1) gene of C. krusei, it was possible to develop a sensitive detection system to both identify and quantitate the level of C. krusei growth in an artificially contaminated apple juice sample.

AB - A real-time PCR system was used to differentiate between the common spoilage yeasts, Zygosaccharomyces bailii, Zygosaccharomyces rouxii, Candida krusei, Rhodotorula glutinis and Saccharomyces cerevisiae, based on melting peak Tm analysis of the 5.8S rDNA subunit and the adjacent ITS2 region of these yeasts. By using the real-time PCR system and by targeting the citrate synthase (cs 1) gene of C. krusei, it was possible to develop a sensitive detection system to both identify and quantitate the level of C. krusei growth in an artificially contaminated apple juice sample.

KW - Candida krusei

KW - Real-time PCR

UR - https://www.scopus.com/pages/publications/1242338163

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JO - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

IS - 3

ER -

Potential of using real-time PCR-based detection of spoilage yeast in fruit juice - A preliminary study

Abstract

Keywords

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