Prevotella rara sp. nov., isolated from human faeces

Boris A. Efimov; Andrei V. Chaplin; Victoria A. Shcherbakova; Natalia E. Suzina; Irina V. Podoprigora; Andrei N. Shkoporov

doi:10.1099/ijsem.0.003066

Prevotella rara sp. nov., isolated from human faeces

Boris A. Efimov
, Andrei V. Chaplin
, Victoria A. Shcherbakova
, Natalia E. Suzina
, Irina V. Podoprigora
, Andrei N. Shkoporov

Research output: Contribution to journal › Article › peer-review

Abstract

A strain of obligately anaerobic, Gram-stain-negative rods was isolated from human faeces and characterized both phenotypically and genotypically. Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed the strain to represent a member of the genus Prevotella, distant from the species with validly published names, with the closest relationship to Prevotella oryzae. The strain was moderately saccharolytic and proteolytic. The predominant menaquinones were MK-13 and MK-12. The major cellular long-chain fatty acids were anteiso-C _{15: 0} and iso-C _{15: 0} . The genomic DNA G+C content was 45.7 mol%. On the basis of chemotaxonomic and genotypic properties, it was concluded that the strain represent a novel species within the genus Prevotella, for which the name Prevotella rara sp. nov. is proposed. The type strain of Prevotella rara is 109 ^T (=VKM B-2992 ^T =DSM 105141 ^T ).

Original language	English
Article number	003066
Pages (from-to)	3818-3825
Number of pages	8
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	68
Issue number	12
DOIs	https://doi.org/10.1099/ijsem.0.003066
Publication status	Published - Dec 2018

Keywords

Bacteroidetes
Microbiota
Novel species
Prevotella

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10.1099/ijsem.0.003066

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title = "Prevotella rara sp. nov., isolated from human faeces",

abstract = " A strain of obligately anaerobic, Gram-stain-negative rods was isolated from human faeces and characterized both phenotypically and genotypically. Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed the strain to represent a member of the genus Prevotella, distant from the species with validly published names, with the closest relationship to Prevotella oryzae. The strain was moderately saccharolytic and proteolytic. The predominant menaquinones were MK-13 and MK-12. The major cellular long-chain fatty acids were anteiso-C 15: 0 and iso-C 15: 0 . The genomic DNA G+C content was 45.7 mol\%. On the basis of chemotaxonomic and genotypic properties, it was concluded that the strain represent a novel species within the genus Prevotella, for which the name Prevotella rara sp. nov. is proposed. The type strain of Prevotella rara is 109 T (=VKM B-2992 T =DSM 105141 T ).",

keywords = "Bacteroidetes, Microbiota, Novel species, Prevotella",

author = "Efimov, \{Boris A.\} and Chaplin, \{Andrei V.\} and Shcherbakova, \{Victoria A.\} and Suzina, \{Natalia E.\} and Podoprigora, \{Irina V.\} and Shkoporov, \{Andrei N.\}",

note = "Publisher Copyright: {\textcopyright} 2018 IUMS.",

year = "2018",

month = dec,

doi = "10.1099/ijsem.0.003066",

language = "English",

volume = "68",

pages = "3818--3825",

journal = "International Journal of Systematic and Evolutionary Microbiology",

issn = "1466-5026",

publisher = "Microbiology Society",

number = "12",

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T1 - Prevotella rara sp. nov., isolated from human faeces

AU - Efimov, Boris A.

AU - Chaplin, Andrei V.

AU - Shcherbakova, Victoria A.

AU - Suzina, Natalia E.

AU - Podoprigora, Irina V.

AU - Shkoporov, Andrei N.

PY - 2018/12

Y1 - 2018/12

N2 - A strain of obligately anaerobic, Gram-stain-negative rods was isolated from human faeces and characterized both phenotypically and genotypically. Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed the strain to represent a member of the genus Prevotella, distant from the species with validly published names, with the closest relationship to Prevotella oryzae. The strain was moderately saccharolytic and proteolytic. The predominant menaquinones were MK-13 and MK-12. The major cellular long-chain fatty acids were anteiso-C 15: 0 and iso-C 15: 0 . The genomic DNA G+C content was 45.7 mol%. On the basis of chemotaxonomic and genotypic properties, it was concluded that the strain represent a novel species within the genus Prevotella, for which the name Prevotella rara sp. nov. is proposed. The type strain of Prevotella rara is 109 T (=VKM B-2992 T =DSM 105141 T ).

AB - A strain of obligately anaerobic, Gram-stain-negative rods was isolated from human faeces and characterized both phenotypically and genotypically. Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed the strain to represent a member of the genus Prevotella, distant from the species with validly published names, with the closest relationship to Prevotella oryzae. The strain was moderately saccharolytic and proteolytic. The predominant menaquinones were MK-13 and MK-12. The major cellular long-chain fatty acids were anteiso-C 15: 0 and iso-C 15: 0 . The genomic DNA G+C content was 45.7 mol%. On the basis of chemotaxonomic and genotypic properties, it was concluded that the strain represent a novel species within the genus Prevotella, for which the name Prevotella rara sp. nov. is proposed. The type strain of Prevotella rara is 109 T (=VKM B-2992 T =DSM 105141 T ).

KW - Bacteroidetes

KW - Microbiota

KW - Novel species

KW - Prevotella

UR - https://www.scopus.com/pages/publications/85059795495

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DO - 10.1099/ijsem.0.003066

M3 - Article

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AN - SCOPUS:85059795495

SN - 1466-5026

VL - 68

SP - 3818

EP - 3825

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

IS - 12

M1 - 003066

ER -

Prevotella rara sp. nov., isolated from human faeces

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Keywords

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