Probing inhibitory effects of nanocrystalline cellulose: Inhibition versus surface charge

Keith B. Male; Alfred C.W. Leung; Johnny Montes; Amine Kamen; John H.T. Luong

doi:10.1039/c2nr11886f

Probing inhibitory effects of nanocrystalline cellulose: Inhibition versus surface charge

Keith B. Male
, Alfred C.W. Leung
, Johnny Montes
, Amine Kamen
, John H.T. Luong

National Research Council of Canada

Research output: Contribution to journal › Article › peer-review

Abstract

NCC derived from different biomass sources was probed for its plausible cytotoxicity by electric cell-substrate impedance sensing (ECIS). Two different cell lines, Spodoptera frugiperda Sf9 insect cells and Chinese hamster lung fibroblast V79, were exposed to NCC and their spreading and viability were monitored and quantified by ECIS. Based on the 50%-inhibition concentration (ECIS ₅₀), none of the NCC produced was judged to have any significant cytotoxicity on these two cell lines. However, NCC derived from flax exhibited the most pronounced inhibition on Sf9 compared to hemp and cellulose powder. NCCs from flax and hemp pre-treated with pectate lyase were also less inhibitory than NCCs prepared from untreated flax and hemp. Results also suggested a correlation between the inhibitory effect and the carboxylic acid contents on the NCC.

Original language	English
Pages (from-to)	1373-1379
Number of pages	7
Journal	Nanoscale
Volume	4
Issue number	4
DOIs	https://doi.org/10.1039/c2nr11886f
Publication status	Published - 21 Feb 2012
Externally published	Yes

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10.1039/c2nr11886f

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title = "Probing inhibitory effects of nanocrystalline cellulose: Inhibition versus surface charge",

abstract = "NCC derived from different biomass sources was probed for its plausible cytotoxicity by electric cell-substrate impedance sensing (ECIS). Two different cell lines, Spodoptera frugiperda Sf9 insect cells and Chinese hamster lung fibroblast V79, were exposed to NCC and their spreading and viability were monitored and quantified by ECIS. Based on the 50\%-inhibition concentration (ECIS 50), none of the NCC produced was judged to have any significant cytotoxicity on these two cell lines. However, NCC derived from flax exhibited the most pronounced inhibition on Sf9 compared to hemp and cellulose powder. NCCs from flax and hemp pre-treated with pectate lyase were also less inhibitory than NCCs prepared from untreated flax and hemp. Results also suggested a correlation between the inhibitory effect and the carboxylic acid contents on the NCC.",

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T2 - Inhibition versus surface charge

AU - Male, Keith B.

AU - Leung, Alfred C.W.

AU - Montes, Johnny

AU - Kamen, Amine

AU - Luong, John H.T.

PY - 2012/2/21

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N2 - NCC derived from different biomass sources was probed for its plausible cytotoxicity by electric cell-substrate impedance sensing (ECIS). Two different cell lines, Spodoptera frugiperda Sf9 insect cells and Chinese hamster lung fibroblast V79, were exposed to NCC and their spreading and viability were monitored and quantified by ECIS. Based on the 50%-inhibition concentration (ECIS 50), none of the NCC produced was judged to have any significant cytotoxicity on these two cell lines. However, NCC derived from flax exhibited the most pronounced inhibition on Sf9 compared to hemp and cellulose powder. NCCs from flax and hemp pre-treated with pectate lyase were also less inhibitory than NCCs prepared from untreated flax and hemp. Results also suggested a correlation between the inhibitory effect and the carboxylic acid contents on the NCC.

AB - NCC derived from different biomass sources was probed for its plausible cytotoxicity by electric cell-substrate impedance sensing (ECIS). Two different cell lines, Spodoptera frugiperda Sf9 insect cells and Chinese hamster lung fibroblast V79, were exposed to NCC and their spreading and viability were monitored and quantified by ECIS. Based on the 50%-inhibition concentration (ECIS 50), none of the NCC produced was judged to have any significant cytotoxicity on these two cell lines. However, NCC derived from flax exhibited the most pronounced inhibition on Sf9 compared to hemp and cellulose powder. NCCs from flax and hemp pre-treated with pectate lyase were also less inhibitory than NCCs prepared from untreated flax and hemp. Results also suggested a correlation between the inhibitory effect and the carboxylic acid contents on the NCC.

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DO - 10.1039/c2nr11886f

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Probing inhibitory effects of nanocrystalline cellulose: Inhibition versus surface charge

Abstract

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