TY - JOUR
T1 - Purification and functional properties of Rab11-FIP3
AU - Horgan, Conor P.
AU - Zurawski, Tomas H.
AU - McCaffrey, Mary W.
PY - 2005
Y1 - 2005
N2 - The Rab family of small GTPases are key regulators of membrane trafficking in eukaryotic cells. Rab11, one member of this family, plays a role in regulating various cellular functions such as plasma membrane recycling, phagocytosis, and cytokinesis. A family of Rab11-binding proteins has been identified and termed the Rab11 family interacting proteins or Rab11-FIPs. Rab11-FIP3, a member of this Rab11-binding protein family, in addition to interacting with Rab11, is also capable of interaction with members of the ADP-Ribosylation Factor (ARF) GTPase family. Here we describe the purification of Rab11-FIP3 and report its biological properties in eukaryotic cells as visualized by immunofluorescence microscopy.
AB - The Rab family of small GTPases are key regulators of membrane trafficking in eukaryotic cells. Rab11, one member of this family, plays a role in regulating various cellular functions such as plasma membrane recycling, phagocytosis, and cytokinesis. A family of Rab11-binding proteins has been identified and termed the Rab11 family interacting proteins or Rab11-FIPs. Rab11-FIP3, a member of this Rab11-binding protein family, in addition to interacting with Rab11, is also capable of interaction with members of the ADP-Ribosylation Factor (ARF) GTPase family. Here we describe the purification of Rab11-FIP3 and report its biological properties in eukaryotic cells as visualized by immunofluorescence microscopy.
UR - https://www.scopus.com/pages/publications/32344450545
U2 - 10.1016/S0076-6879(05)03044-2
DO - 10.1016/S0076-6879(05)03044-2
M3 - Review article
C2 - 16473615
AN - SCOPUS:32344450545
SN - 0076-6879
VL - 403
SP - 499
EP - 512
JO - Methods in Enzymology
JF - Methods in Enzymology
M1 - 44
ER -