Purification and functional properties of Rab11-FIP3

Conor P. Horgan; Tomas H. Zurawski; Mary W. McCaffrey

doi:10.1016/S0076-6879(05)03044-2

Purification and functional properties of Rab11-FIP3

Conor P. Horgan
, Tomas H. Zurawski
, Mary W. McCaffrey

School of Biochemistry and Cell Biology

Dublin City University

Research output: Contribution to journal › Review article › peer-review

Abstract

The Rab family of small GTPases are key regulators of membrane trafficking in eukaryotic cells. Rab11, one member of this family, plays a role in regulating various cellular functions such as plasma membrane recycling, phagocytosis, and cytokinesis. A family of Rab11-binding proteins has been identified and termed the Rab11 family interacting proteins or Rab11-FIPs. Rab11-FIP3, a member of this Rab11-binding protein family, in addition to interacting with Rab11, is also capable of interaction with members of the ADP-Ribosylation Factor (ARF) GTPase family. Here we describe the purification of Rab11-FIP3 and report its biological properties in eukaryotic cells as visualized by immunofluorescence microscopy.

Original language	English
Article number	44
Pages (from-to)	499-512
Number of pages	14
Journal	Methods in Enzymology
Volume	403
DOIs	https://doi.org/10.1016/S0076-6879(05)03044-2
Publication status	Published - 2005

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title = "Purification and functional properties of Rab11-FIP3",

abstract = "The Rab family of small GTPases are key regulators of membrane trafficking in eukaryotic cells. Rab11, one member of this family, plays a role in regulating various cellular functions such as plasma membrane recycling, phagocytosis, and cytokinesis. A family of Rab11-binding proteins has been identified and termed the Rab11 family interacting proteins or Rab11-FIPs. Rab11-FIP3, a member of this Rab11-binding protein family, in addition to interacting with Rab11, is also capable of interaction with members of the ADP-Ribosylation Factor (ARF) GTPase family. Here we describe the purification of Rab11-FIP3 and report its biological properties in eukaryotic cells as visualized by immunofluorescence microscopy.",

author = "Horgan, \{Conor P.\} and Zurawski, \{Tomas H.\} and McCaffrey, \{Mary W.\}",

year = "2005",

doi = "10.1016/S0076-6879(05)03044-2",

language = "English",

volume = "403",

pages = "499--512",

journal = "Methods in Enzymology",

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AU - Horgan, Conor P.

AU - Zurawski, Tomas H.

AU - McCaffrey, Mary W.

PY - 2005

Y1 - 2005

N2 - The Rab family of small GTPases are key regulators of membrane trafficking in eukaryotic cells. Rab11, one member of this family, plays a role in regulating various cellular functions such as plasma membrane recycling, phagocytosis, and cytokinesis. A family of Rab11-binding proteins has been identified and termed the Rab11 family interacting proteins or Rab11-FIPs. Rab11-FIP3, a member of this Rab11-binding protein family, in addition to interacting with Rab11, is also capable of interaction with members of the ADP-Ribosylation Factor (ARF) GTPase family. Here we describe the purification of Rab11-FIP3 and report its biological properties in eukaryotic cells as visualized by immunofluorescence microscopy.

AB - The Rab family of small GTPases are key regulators of membrane trafficking in eukaryotic cells. Rab11, one member of this family, plays a role in regulating various cellular functions such as plasma membrane recycling, phagocytosis, and cytokinesis. A family of Rab11-binding proteins has been identified and termed the Rab11 family interacting proteins or Rab11-FIPs. Rab11-FIP3, a member of this Rab11-binding protein family, in addition to interacting with Rab11, is also capable of interaction with members of the ADP-Ribosylation Factor (ARF) GTPase family. Here we describe the purification of Rab11-FIP3 and report its biological properties in eukaryotic cells as visualized by immunofluorescence microscopy.

UR - https://www.scopus.com/pages/publications/32344450545

U2 - 10.1016/S0076-6879(05)03044-2

DO - 10.1016/S0076-6879(05)03044-2

M3 - Review article

C2 - 16473615

AN - SCOPUS:32344450545

SN - 0076-6879

VL - 403

SP - 499

EP - 512

JO - Methods in Enzymology

JF - Methods in Enzymology

M1 - 44

ER -

Purification and functional properties of Rab11-FIP3

Abstract

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