Rapid electrochemical detection of pseudomonas aeruginosa signaling molecules by boron-doped diamond electrode

Alyah Buzid; John H.T. Luong; F. Jerry Reen; Fergal O’Gara; Jeremy D. Glennon; Gerard P. McGlacken

doi:10.1007/978-1-4939-7309-5_9

Rapid electrochemical detection of pseudomonas aeruginosa signaling molecules by boron-doped diamond electrode

Alyah Buzid
, John H.T. Luong
, F. Jerry Reen
, Fergal O’Gara
, Jeremy D. Glennon
, Gerard P. McGlacken

Innovative Chromatography Group
Curtin University

Research output: Chapter in Book/Report/Conference proceedings › Chapter › peer-review

Abstract

As the leading cause of morbidity and mortality of cystic fibrosis (CF) patients, early detection of Pseudomonas aeruginosa (PA) is critical in the clinical management of this pathogen. Herein, we describe rapid and sensitive electroanalytical methods using differential pulse voltammetry (DPV) at a boron-doped diamond (BDD) electrode for the detection of PA signaling biomolecules. Monitoring the production of key signaling molecules in bacterial cultures of P. aeruginosa PA14 over 8Â h is described, involving sample pretreatment by liquid-liquid and solid-phase extraction. In addition, direct electrochemical detection approach of PA signaling molecules is also reported in conjunction with hexadecyltrimethylammonium bromide (CTAB) to disrupt the bacterial membrane.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	107-116
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-4939-7309-5_9
Publication status	Published - 2018

Publication series

Name	Methods in Molecular Biology
Volume	1673
ISSN (Print)	1064-3745

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Boron-doped diamond electrode
Cationic surfactants
Cystic fibrosis
Electrochemical detection
Extraction
Pseudomonas aeruginosa
Signaling molecules

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10.1007/978-1-4939-7309-5_9

Cite this

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title = "Rapid electrochemical detection of pseudomonas aeruginosa signaling molecules by boron-doped diamond electrode",

abstract = "As the leading cause of morbidity and mortality of cystic fibrosis (CF) patients, early detection of Pseudomonas aeruginosa (PA) is critical in the clinical management of this pathogen. Herein, we describe rapid and sensitive electroanalytical methods using differential pulse voltammetry (DPV) at a boron-doped diamond (BDD) electrode for the detection of PA signaling biomolecules. Monitoring the production of key signaling molecules in bacterial cultures of P. aeruginosa PA14 over 8{\^A} h is described, involving sample pretreatment by liquid-liquid and solid-phase extraction. In addition, direct electrochemical detection approach of PA signaling molecules is also reported in conjunction with hexadecyltrimethylammonium bromide (CTAB) to disrupt the bacterial membrane.",

keywords = "Boron-doped diamond electrode, Cationic surfactants, Cystic fibrosis, Electrochemical detection, Extraction, Pseudomonas aeruginosa, Signaling molecules",

author = "Alyah Buzid and Luong, \{John H.T.\} and Reen, \{F. Jerry\} and Fergal O{\textquoteright}Gara and Glennon, \{Jeremy D.\} and McGlacken, \{Gerard P.\}",

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year = "2018",

doi = "10.1007/978-1-4939-7309-5\_9",

language = "English",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc.",

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TY - CHAP

T1 - Rapid electrochemical detection of pseudomonas aeruginosa signaling molecules by boron-doped diamond electrode

AU - Buzid, Alyah

AU - Luong, John H.T.

AU - Reen, F. Jerry

AU - O’Gara, Fergal

AU - Glennon, Jeremy D.

AU - McGlacken, Gerard P.

PY - 2018

Y1 - 2018

N2 - As the leading cause of morbidity and mortality of cystic fibrosis (CF) patients, early detection of Pseudomonas aeruginosa (PA) is critical in the clinical management of this pathogen. Herein, we describe rapid and sensitive electroanalytical methods using differential pulse voltammetry (DPV) at a boron-doped diamond (BDD) electrode for the detection of PA signaling biomolecules. Monitoring the production of key signaling molecules in bacterial cultures of P. aeruginosa PA14 over 8Â h is described, involving sample pretreatment by liquid-liquid and solid-phase extraction. In addition, direct electrochemical detection approach of PA signaling molecules is also reported in conjunction with hexadecyltrimethylammonium bromide (CTAB) to disrupt the bacterial membrane.

AB - As the leading cause of morbidity and mortality of cystic fibrosis (CF) patients, early detection of Pseudomonas aeruginosa (PA) is critical in the clinical management of this pathogen. Herein, we describe rapid and sensitive electroanalytical methods using differential pulse voltammetry (DPV) at a boron-doped diamond (BDD) electrode for the detection of PA signaling biomolecules. Monitoring the production of key signaling molecules in bacterial cultures of P. aeruginosa PA14 over 8Â h is described, involving sample pretreatment by liquid-liquid and solid-phase extraction. In addition, direct electrochemical detection approach of PA signaling molecules is also reported in conjunction with hexadecyltrimethylammonium bromide (CTAB) to disrupt the bacterial membrane.

KW - Boron-doped diamond electrode

KW - Cationic surfactants

KW - Cystic fibrosis

KW - Electrochemical detection

KW - Extraction

KW - Pseudomonas aeruginosa

KW - Signaling molecules

UR - https://www.scopus.com/pages/publications/85033668940

U2 - 10.1007/978-1-4939-7309-5_9

DO - 10.1007/978-1-4939-7309-5_9

M3 - Chapter

C2 - 29130168

AN - SCOPUS:85033668940

T3 - Methods in Molecular Biology

SP - 107

EP - 116

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Rapid electrochemical detection of pseudomonas aeruginosa signaling molecules by boron-doped diamond electrode

Abstract

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UN SDGs

Keywords

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