Rapid Nanomolar Detection of Guaiacol from its Precursors Using a Core-shell Reversed-phase Column Coupled with a Boron-doped Diamond Electrode

Phyllis E. Hayes; Alyah Buzid; John H.T. Luong; Jeremy D. Glennon

doi:10.1002/elan.202060434

Rapid Nanomolar Detection of Guaiacol from its Precursors Using a Core-shell Reversed-phase Column Coupled with a Boron-doped Diamond Electrode

Phyllis E. Hayes
, Alyah Buzid
, John H.T. Luong
, Jeremy D. Glennon

School of Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

The electrooxidation mechanisms of guaiacol (2-methoxyphenol), a food and beverage spoilage metabolite, and its precursors; vanillic acid (VA), vanillin, and ferulic acid (FA) were investigated by cyclic voltammetry (CV) with a boron-doped diamond (BDD) electrode. Reversed-phase liquid chromatography (RPLC) together with a BDD electrode poised at +1.6 V vs. Pd/H₂, was optimized for their sensitive detection. The separation was achieved in 60 s with a core-shell column (HALO C₁₈). The detection limits of these analytes ranged from 10–30 nM. The method was applicable for the analysis of guaiacol and its precursors from a popular commercial drink.

Original language	English
Pages (from-to)	766-773
Number of pages	8
Journal	Electroanalysis
Volume	33
Issue number	3
DOIs	https://doi.org/10.1002/elan.202060434
Publication status	Published - Mar 2021

Keywords

Alicyclobacillus spp
Boron-doped diamond electrode
Core-shell column
Guaiacol
High-performance liquid chromatography

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10.1002/elan.202060434

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title = "Rapid Nanomolar Detection of Guaiacol from its Precursors Using a Core-shell Reversed-phase Column Coupled with a Boron-doped Diamond Electrode",

abstract = "The electrooxidation mechanisms of guaiacol (2-methoxyphenol), a food and beverage spoilage metabolite, and its precursors; vanillic acid (VA), vanillin, and ferulic acid (FA) were investigated by cyclic voltammetry (CV) with a boron-doped diamond (BDD) electrode. Reversed-phase liquid chromatography (RPLC) together with a BDD electrode poised at +1.6 V vs. Pd/H2, was optimized for their sensitive detection. The separation was achieved in 60 s with a core-shell column (HALO C18). The detection limits of these analytes ranged from 10–30 nM. The method was applicable for the analysis of guaiacol and its precursors from a popular commercial drink.",

keywords = "Alicyclobacillus spp, Boron-doped diamond electrode, Core-shell column, Guaiacol, High-performance liquid chromatography",

author = "Hayes, \{Phyllis E.\} and Alyah Buzid and Luong, \{John H.T.\} and Glennon, \{Jeremy D.\}",

note = "Publisher Copyright: {\textcopyright} 2020 Wiley-VCH GmbH",

year = "2021",

month = mar,

doi = "10.1002/elan.202060434",

language = "English",

volume = "33",

pages = "766--773",

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AU - Hayes, Phyllis E.

AU - Buzid, Alyah

AU - Luong, John H.T.

AU - Glennon, Jeremy D.

PY - 2021/3

Y1 - 2021/3

N2 - The electrooxidation mechanisms of guaiacol (2-methoxyphenol), a food and beverage spoilage metabolite, and its precursors; vanillic acid (VA), vanillin, and ferulic acid (FA) were investigated by cyclic voltammetry (CV) with a boron-doped diamond (BDD) electrode. Reversed-phase liquid chromatography (RPLC) together with a BDD electrode poised at +1.6 V vs. Pd/H2, was optimized for their sensitive detection. The separation was achieved in 60 s with a core-shell column (HALO C18). The detection limits of these analytes ranged from 10–30 nM. The method was applicable for the analysis of guaiacol and its precursors from a popular commercial drink.

AB - The electrooxidation mechanisms of guaiacol (2-methoxyphenol), a food and beverage spoilage metabolite, and its precursors; vanillic acid (VA), vanillin, and ferulic acid (FA) were investigated by cyclic voltammetry (CV) with a boron-doped diamond (BDD) electrode. Reversed-phase liquid chromatography (RPLC) together with a BDD electrode poised at +1.6 V vs. Pd/H2, was optimized for their sensitive detection. The separation was achieved in 60 s with a core-shell column (HALO C18). The detection limits of these analytes ranged from 10–30 nM. The method was applicable for the analysis of guaiacol and its precursors from a popular commercial drink.

KW - Alicyclobacillus spp

KW - Boron-doped diamond electrode

KW - Core-shell column

KW - Guaiacol

KW - High-performance liquid chromatography

UR - https://www.scopus.com/pages/publications/85096685921

U2 - 10.1002/elan.202060434

DO - 10.1002/elan.202060434

M3 - Article

AN - SCOPUS:85096685921

SN - 1040-0397

VL - 33

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EP - 773

JO - Electroanalysis

JF - Electroanalysis

IS - 3

ER -

Rapid Nanomolar Detection of Guaiacol from its Precursors Using a Core-shell Reversed-phase Column Coupled with a Boron-doped Diamond Electrode

Abstract

Keywords

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