TY - JOUR
T1 - Redox proteomics reveals the hepatoprotective effect of must from Pedro Ximénez dried grapes in aged Mus spretus mice
AU - Morales-Prieto, Noelia
AU - López de Lerma, Nieves
AU - Pacheco, Isabel L.
AU - Pérez, José
AU - Peinado, Rafael A.
AU - Abril, Nieves
N1 - Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017/7/1
Y1 - 2017/7/1
N2 - Aged mice consumed water supplemented with Pedro Ximénez must (PXM). After 20 weeks, histological analyses showed that PXM consumption highly alleviated the age-related hepatic damage observed in control mice, restoring the cytoplasmic appearance and normal arrangement of hepatic cords of young mice. Bidimensional electrophoresis of 5-IAF labeled hepatic proteins showed changes in the reversibly oxidized thiol status of 42 protein spots. Fourteen proteins were identified. Seven (FAM210B, RPE, SRSF1, KRT1, ATF1, ZEB1, IgM) resulted reduced and other seven (RPL29, GABPB1, ACTB, DDC, INA, PRKAR1B, ZFP738) oxidized. For 6 proteins (ATF1, RPE, ZEB1, IGM, PRKAR1B and ZFP738) the susceptibility of a particular Cys to reversible oxidation was analyze. The histological changes, the variations in the oxidation status of protein thiolic groups and the identification of specific Cys residues affected, indicated that the PXM must is acting as an antioxidant but also a pro-oxidant that might help the elderly liver to regenerate.
AB - Aged mice consumed water supplemented with Pedro Ximénez must (PXM). After 20 weeks, histological analyses showed that PXM consumption highly alleviated the age-related hepatic damage observed in control mice, restoring the cytoplasmic appearance and normal arrangement of hepatic cords of young mice. Bidimensional electrophoresis of 5-IAF labeled hepatic proteins showed changes in the reversibly oxidized thiol status of 42 protein spots. Fourteen proteins were identified. Seven (FAM210B, RPE, SRSF1, KRT1, ATF1, ZEB1, IgM) resulted reduced and other seven (RPL29, GABPB1, ACTB, DDC, INA, PRKAR1B, ZFP738) oxidized. For 6 proteins (ATF1, RPE, ZEB1, IGM, PRKAR1B and ZFP738) the susceptibility of a particular Cys to reversible oxidation was analyze. The histological changes, the variations in the oxidation status of protein thiolic groups and the identification of specific Cys residues affected, indicated that the PXM must is acting as an antioxidant but also a pro-oxidant that might help the elderly liver to regenerate.
KW - Aging
KW - Liver
KW - Mus spretus mice
KW - Pedro Ximénez dried grapes
KW - Polyphenols redox proteomic
UR - https://www.scopus.com/pages/publications/85018529088
U2 - 10.1016/j.jff.2017.04.025
DO - 10.1016/j.jff.2017.04.025
M3 - Article
AN - SCOPUS:85018529088
SN - 1756-4646
VL - 34
SP - 89
EP - 97
JO - Journal of Functional Foods
JF - Journal of Functional Foods
ER -