TY - JOUR
T1 - Regulated expression of the dinR and recA genes during competence development and SOS induction in Bacillus subtilis
AU - Haijema, Bert Jan
AU - Van Sinderen, Douwe
AU - Winterling, Kevin
AU - Kooistra, Jan
AU - Venema, Gerard
AU - Hamoen, Leendert Willem
PY - 1996
Y1 - 1996
N2 - It has been hypothesized that the dinR gene product of Bacillus subtilis acts as a repressor of the SOS regulon by binding to DNA sequences located upstream of SOS genes, including dinR and recA. Following activation as a result of DNA damage, RecA is believed to catalyse DinR-autocleavage, thus derepressing the SOS regulon. The present results support this hypothesis: a dinR insertion mutation caused a high, constitutive expression of both dinR and recA, which could not be further elevated by SOS-induction. In addition, gel-retardation assays demonstrated a direct interaction between the dinR gene product and the recA and dinR promoter regions. Epistatic interactions and gel-retardation assays demonstrated that the previously reported competence-specific expression of recA directly depended upon the gene product of comK, the competence transcription factor. These data demonstrate the existence of a direct regulatory link between the competence signal-transduction pathway and the SOS regulon.
AB - It has been hypothesized that the dinR gene product of Bacillus subtilis acts as a repressor of the SOS regulon by binding to DNA sequences located upstream of SOS genes, including dinR and recA. Following activation as a result of DNA damage, RecA is believed to catalyse DinR-autocleavage, thus derepressing the SOS regulon. The present results support this hypothesis: a dinR insertion mutation caused a high, constitutive expression of both dinR and recA, which could not be further elevated by SOS-induction. In addition, gel-retardation assays demonstrated a direct interaction between the dinR gene product and the recA and dinR promoter regions. Epistatic interactions and gel-retardation assays demonstrated that the previously reported competence-specific expression of recA directly depended upon the gene product of comK, the competence transcription factor. These data demonstrate the existence of a direct regulatory link between the competence signal-transduction pathway and the SOS regulon.
UR - https://www.scopus.com/pages/publications/0029835935
U2 - 10.1111/j.1365-2958.1996.tb02657.x
DO - 10.1111/j.1365-2958.1996.tb02657.x
M3 - Article
C2 - 8899710
AN - SCOPUS:0029835935
SN - 0950-382X
VL - 22
SP - 75
EP - 85
JO - Molecular Microbiology
JF - Molecular Microbiology
IS - 1
ER -