Regulation of polyphosphate kinase gene expression in Acinetobacter baumannii 252

Julie Ann Gavigan; Leonard M. Marshall; Alan D.W. Dobson

doi:10.1099/00221287-145-10-2931

Regulation of polyphosphate kinase gene expression in Acinetobacter baumannii 252

Julie Ann Gavigan
, Leonard M. Marshall
, Alan D.W. Dobson

University College Cork

Research output: Contribution to journal › Article › peer-review

Abstract

A strain of Acinetobacter baumannii cultured in butyric acid media was found to take up phosphate following a period of phosphate release. PCR was used to clone the polyphosphate kinase (ppk) gene from the strain. The promoter for the ppk gene was functional in the heterologous Escherichia coli host. Using RT-PCR, transcription of the ppk gene was found to be regulated by phosphate concentration.

Original language	English
Pages (from-to)	2931-2937
Number of pages	7
Journal	Microbiology (United Kingdom)
Volume	145
Issue number	10
DOIs	https://doi.org/10.1099/00221287-145-10-2931
Publication status	Published - Oct 1999

Keywords

Acinetobacter baumannii
Polyphosphate kinase
RT-PCR

Access to Document

10.1099/00221287-145-10-2931

Cite this

@article{e8e21d37653749ea8af4405ad7f34b25,

title = "Regulation of polyphosphate kinase gene expression in Acinetobacter baumannii 252",

abstract = "A strain of Acinetobacter baumannii cultured in butyric acid media was found to take up phosphate following a period of phosphate release. PCR was used to clone the polyphosphate kinase (ppk) gene from the strain. The promoter for the ppk gene was functional in the heterologous Escherichia coli host. Using RT-PCR, transcription of the ppk gene was found to be regulated by phosphate concentration.",

keywords = "Acinetobacter baumannii, Polyphosphate kinase, RT-PCR",

author = "Gavigan, \{Julie Ann\} and Marshall, \{Leonard M.\} and Dobson, \{Alan D.W.\}",

year = "1999",

month = oct,

doi = "10.1099/00221287-145-10-2931",

language = "English",

volume = "145",

pages = "2931--2937",

journal = "Microbiology (United Kingdom)",

issn = "1350-0872",

publisher = "Microbiology Society",

number = "10",

}

TY - JOUR

T1 - Regulation of polyphosphate kinase gene expression in Acinetobacter baumannii 252

AU - Gavigan, Julie Ann

AU - Marshall, Leonard M.

AU - Dobson, Alan D.W.

PY - 1999/10

Y1 - 1999/10

N2 - A strain of Acinetobacter baumannii cultured in butyric acid media was found to take up phosphate following a period of phosphate release. PCR was used to clone the polyphosphate kinase (ppk) gene from the strain. The promoter for the ppk gene was functional in the heterologous Escherichia coli host. Using RT-PCR, transcription of the ppk gene was found to be regulated by phosphate concentration.

AB - A strain of Acinetobacter baumannii cultured in butyric acid media was found to take up phosphate following a period of phosphate release. PCR was used to clone the polyphosphate kinase (ppk) gene from the strain. The promoter for the ppk gene was functional in the heterologous Escherichia coli host. Using RT-PCR, transcription of the ppk gene was found to be regulated by phosphate concentration.

KW - Acinetobacter baumannii

KW - Polyphosphate kinase

KW - RT-PCR

UR - https://www.scopus.com/pages/publications/0032874024

U2 - 10.1099/00221287-145-10-2931

DO - 10.1099/00221287-145-10-2931

M3 - Article

C2 - 10537215

AN - SCOPUS:0032874024

SN - 1350-0872

VL - 145

SP - 2931

EP - 2937

JO - Microbiology (United Kingdom)

JF - Microbiology (United Kingdom)

IS - 10

ER -

Regulation of polyphosphate kinase gene expression in Acinetobacter baumannii 252

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this