Relationship between suppression of E6 and E7 virus oncogenes and expression of apoptosis and cell cycle genes in cervical cancer culture

E. V. Khokhlova; A. N. Shkoporov; N. N. Volodin; B. A. Efimov; K. A. Pavlov; L. I. Kafarskaia

doi:10.1007/s10517-010-0879-2

Relationship between suppression of E6 and E7 virus oncogenes and expression of apoptosis and cell cycle genes in cervical cancer culture

E. V. Khokhlova
, A. N. Shkoporov
, N. N. Volodin
, B. A. Efimov
, K. A. Pavlov
, L. I. Kafarskaia

Pirogov Russian National Research Medical University

Research output: Contribution to journal › Article › peer-review

Abstract

The effects of short interfering RNA suppressing the expression of E6 and E7 human papilloma virus (type 18) on the expression of apoptosis and cell cycle genes were studied in HeLa cells. Changes in the transcription profiles were evaluated using DNA microarray and real-time reverse-transcription PCR. Cell transfection with anti-E6 and anti-E7 short interfering RNA moderately reduced the expression of mRNA for CDC25C, GRB2, GTSE1, and PLK1 genes and induced expression of CDKN1A (p21^CIP) gene mRNA. In addition, culture proliferation was inhibited and morphological changes characteristic of differentiation and cell aging developed.

Original language	English
Pages (from-to)	73-77
Number of pages	5
Journal	Bulletin of Experimental Biology and Medicine
Volume	149
Issue number	1
DOIs	https://doi.org/10.1007/s10517-010-0879-2
Publication status	Published - Jul 2010
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

human papilloma viruses
RNA interference
short interfering RNA
viral Oncogenes

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10.1007/s10517-010-0879-2

Cite this

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title = "Relationship between suppression of E6 and E7 virus oncogenes and expression of apoptosis and cell cycle genes in cervical cancer culture",

abstract = "The effects of short interfering RNA suppressing the expression of E6 and E7 human papilloma virus (type 18) on the expression of apoptosis and cell cycle genes were studied in HeLa cells. Changes in the transcription profiles were evaluated using DNA microarray and real-time reverse-transcription PCR. Cell transfection with anti-E6 and anti-E7 short interfering RNA moderately reduced the expression of mRNA for CDC25C, GRB2, GTSE1, and PLK1 genes and induced expression of CDKN1A (p21CIP) gene mRNA. In addition, culture proliferation was inhibited and morphological changes characteristic of differentiation and cell aging developed.",

keywords = "human papilloma viruses, RNA interference, short interfering RNA, viral Oncogenes",

author = "Khokhlova, \{E. V.\} and Shkoporov, \{A. N.\} and Volodin, \{N. N.\} and Efimov, \{B. A.\} and Pavlov, \{K. A.\} and Kafarskaia, \{L. I.\}",

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doi = "10.1007/s10517-010-0879-2",

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TY - JOUR

T1 - Relationship between suppression of E6 and E7 virus oncogenes and expression of apoptosis and cell cycle genes in cervical cancer culture

AU - Khokhlova, E. V.

AU - Shkoporov, A. N.

AU - Volodin, N. N.

AU - Efimov, B. A.

AU - Pavlov, K. A.

AU - Kafarskaia, L. I.

PY - 2010/7

Y1 - 2010/7

N2 - The effects of short interfering RNA suppressing the expression of E6 and E7 human papilloma virus (type 18) on the expression of apoptosis and cell cycle genes were studied in HeLa cells. Changes in the transcription profiles were evaluated using DNA microarray and real-time reverse-transcription PCR. Cell transfection with anti-E6 and anti-E7 short interfering RNA moderately reduced the expression of mRNA for CDC25C, GRB2, GTSE1, and PLK1 genes and induced expression of CDKN1A (p21CIP) gene mRNA. In addition, culture proliferation was inhibited and morphological changes characteristic of differentiation and cell aging developed.

AB - The effects of short interfering RNA suppressing the expression of E6 and E7 human papilloma virus (type 18) on the expression of apoptosis and cell cycle genes were studied in HeLa cells. Changes in the transcription profiles were evaluated using DNA microarray and real-time reverse-transcription PCR. Cell transfection with anti-E6 and anti-E7 short interfering RNA moderately reduced the expression of mRNA for CDC25C, GRB2, GTSE1, and PLK1 genes and induced expression of CDKN1A (p21CIP) gene mRNA. In addition, culture proliferation was inhibited and morphological changes characteristic of differentiation and cell aging developed.

KW - human papilloma viruses

KW - RNA interference

KW - short interfering RNA

KW - viral Oncogenes

UR - https://www.scopus.com/pages/publications/77954427002

U2 - 10.1007/s10517-010-0879-2

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M3 - Article

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AN - SCOPUS:77954427002

SN - 0007-4888

VL - 149

SP - 73

EP - 77

JO - Bulletin of Experimental Biology and Medicine

JF - Bulletin of Experimental Biology and Medicine

IS - 1

ER -

Relationship between suppression of E6 and E7 virus oncogenes and expression of apoptosis and cell cycle genes in cervical cancer culture

Abstract

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Keywords

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