TY - JOUR
T1 - ‘Reliability of new poly (Lactic-co-glycolic acid) membranes treated with oxygen plasma plus silicon dioxide layers for pre-prosthetic guided bone regeneration processes’
AU - Castillo-Dalí, Gabriel
AU - Castillo-Oyagüe, Raquel
AU - Batista-Cruzado, Antonio
AU - López-Santos, Carmen
AU - Rodríguez-González-Elipe, Agustín
AU - Saffar, Jean Louis
AU - Lynch, Christopher D.
AU - Gutiérrez-Pérez, José Luis
AU - Torres-Lagares, Daniel
N1 - Publisher Copyright:
© Medicina Oral S. L. C.I.F.
PY - 2017
Y1 - 2017
N2 - Background: The use of cold plasmas may improve the surface roughness of poly(lactic-co-glycolic) acid (PLGA) membranes, which may stimulate the adhesion of osteogenic mediators and cells, thus accelerating the biodegradation of the barriers. Moreover, the incorporation of metallic-oxide particles to the surface of these membranes may enhance their osteoinductive capacity. Therefore, the aim of this paper was to evaluate the reliability of a new PLGA membrane after being treated with oxygen plasma (PO2) plus silicon dioxide (SiO2) layers for guided bone regeneration (GBR) processes. Material and Methods: Circumferential bone defects (diameter: 11 mm; depth: 3 mm) were created on the top of eight experimentation rabbits’ skulls and were randomly covered with: (1) PLGA membranes (control), or (2) PLGA/ PO2/SiO2 barriers. The animals were euthanized two months afterwards. A micromorphologic study was then performed using ROI (region of interest) colour analysis. Percentage of new bone formation, length of mineralised bone, concentration of osteoclasts, and intensity of ostheosynthetic activity were assessed and compared with those of the original bone tissue. The Kruskal-Wallis test was applied for between-group com asignificance level of a=0.05 was considered. Results: The PLGA/PO2/SiO2 membranes achieved the significantly highest new bone formation, length of miner- alised bone, concentration of osteoclasts, and ostheosynthetic activity. The percentage of regenerated bone supplied by the new membranes was similar to that of the original bone tissue. Unlike what happened in the control group, PLGA/PO2/SiO2 membranes predominantly showed bone layers in advanced stages of formation. Conclusions: The addition of SiO2 layers to PLGA membranes pre-treated with PO2 improves their bone-regeneration potential. Although further research is necessary to corroborate these conclusions in humans, this could be a promising strategy to rebuild the bone architecture prior to rehabilitate edentulous areas.
AB - Background: The use of cold plasmas may improve the surface roughness of poly(lactic-co-glycolic) acid (PLGA) membranes, which may stimulate the adhesion of osteogenic mediators and cells, thus accelerating the biodegradation of the barriers. Moreover, the incorporation of metallic-oxide particles to the surface of these membranes may enhance their osteoinductive capacity. Therefore, the aim of this paper was to evaluate the reliability of a new PLGA membrane after being treated with oxygen plasma (PO2) plus silicon dioxide (SiO2) layers for guided bone regeneration (GBR) processes. Material and Methods: Circumferential bone defects (diameter: 11 mm; depth: 3 mm) were created on the top of eight experimentation rabbits’ skulls and were randomly covered with: (1) PLGA membranes (control), or (2) PLGA/ PO2/SiO2 barriers. The animals were euthanized two months afterwards. A micromorphologic study was then performed using ROI (region of interest) colour analysis. Percentage of new bone formation, length of mineralised bone, concentration of osteoclasts, and intensity of ostheosynthetic activity were assessed and compared with those of the original bone tissue. The Kruskal-Wallis test was applied for between-group com asignificance level of a=0.05 was considered. Results: The PLGA/PO2/SiO2 membranes achieved the significantly highest new bone formation, length of miner- alised bone, concentration of osteoclasts, and ostheosynthetic activity. The percentage of regenerated bone supplied by the new membranes was similar to that of the original bone tissue. Unlike what happened in the control group, PLGA/PO2/SiO2 membranes predominantly showed bone layers in advanced stages of formation. Conclusions: The addition of SiO2 layers to PLGA membranes pre-treated with PO2 improves their bone-regeneration potential. Although further research is necessary to corroborate these conclusions in humans, this could be a promising strategy to rebuild the bone architecture prior to rehabilitate edentulous areas.
KW - Guided bone regeneration (GBR)
KW - Membrane
KW - Nanocomposite
KW - Oxygen plasma (PO)
KW - Poly(lactic-co-glycolic acid) (PLGA)
KW - Silicon dioxide layers
UR - https://www.scopus.com/pages/publications/85014688979
U2 - 10.4317/medoral.21512
DO - 10.4317/medoral.21512
M3 - Article
C2 - 28160588
AN - SCOPUS:85014688979
SN - 1698-4447
VL - 22
SP - e242-e250
JO - Medicina Oral Patologia Oral y Cirugia Bucal
JF - Medicina Oral Patologia Oral y Cirugia Bucal
IS - 2
M1 - 21512
ER -