Ribose utilization by the human commensal Bifidobacterium breve UCC2003

Karina Pokusaeva; Ana Rute Neves; Aldert Zomer; Mary O'Connell-Motherway; John MacSharry; Peter Curley; Gerald F. Fitzgerald; Douwe Van Sinderen

doi:10.1111/j.1751-7915.2009.00152.x

Ribose utilization by the human commensal Bifidobacterium breve UCC2003

Karina Pokusaeva
, Ana Rute Neves
, Aldert Zomer
, Mary O'Connell-Motherway
, John MacSharry
, Peter Curley
, Gerald F. Fitzgerald
, Douwe Van Sinderen

University College Cork
NOVA University Lisbon

Research output: Contribution to journal › Article › peer-review

Abstract

Growth of Bifidobacterium breve UCC2003 on ribose leads to the transcriptional induction of the rbsACBDK gene cluster. Generation and phenotypic analysis of an rbsA insertion mutant established that the rbs gene cluster is essential for ribose utilization, and that its transcription is likely regulated by a LacI-type regulator encoded by rbsR, located immediately upstream of rbsA. Gel mobility shift assays using purified RbsR_His indicate that the promoter upstream of rbsABCDK is negatively controlled by RbsR_His binding to an 18 bp inverted repeat and that RbsR _His binding activity is modulated by D-ribose. The rbsK gene of the rbs operon of B. breve UCC2003 was shown to specify a ribokinase (EC 2.7.1.15), which specifically directs its phosphorylating activity towards D-ribose, converting this pentose sugar to ribose-5-phosphate.

Original language	English
Pages (from-to)	311-323
Number of pages	13
Journal	Microbial Biotechnology
Volume	3
Issue number	3
DOIs	https://doi.org/10.1111/j.1751-7915.2009.00152.x
Publication status	Published - May 2010

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title = "Ribose utilization by the human commensal Bifidobacterium breve UCC2003",

abstract = "Growth of Bifidobacterium breve UCC2003 on ribose leads to the transcriptional induction of the rbsACBDK gene cluster. Generation and phenotypic analysis of an rbsA insertion mutant established that the rbs gene cluster is essential for ribose utilization, and that its transcription is likely regulated by a LacI-type regulator encoded by rbsR, located immediately upstream of rbsA. Gel mobility shift assays using purified RbsRHis indicate that the promoter upstream of rbsABCDK is negatively controlled by RbsRHis binding to an 18 bp inverted repeat and that RbsR His binding activity is modulated by D-ribose. The rbsK gene of the rbs operon of B. breve UCC2003 was shown to specify a ribokinase (EC 2.7.1.15), which specifically directs its phosphorylating activity towards D-ribose, converting this pentose sugar to ribose-5-phosphate.",

author = "Karina Pokusaeva and Neves, \{Ana Rute\} and Aldert Zomer and Mary O'Connell-Motherway and John MacSharry and Peter Curley and Fitzgerald, \{Gerald F.\} and \{Van Sinderen\}, Douwe",

year = "2010",

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doi = "10.1111/j.1751-7915.2009.00152.x",

language = "English",

volume = "3",

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T1 - Ribose utilization by the human commensal Bifidobacterium breve UCC2003

AU - Pokusaeva, Karina

AU - Neves, Ana Rute

AU - Zomer, Aldert

AU - O'Connell-Motherway, Mary

AU - MacSharry, John

AU - Curley, Peter

AU - Fitzgerald, Gerald F.

AU - Van Sinderen, Douwe

PY - 2010/5

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N2 - Growth of Bifidobacterium breve UCC2003 on ribose leads to the transcriptional induction of the rbsACBDK gene cluster. Generation and phenotypic analysis of an rbsA insertion mutant established that the rbs gene cluster is essential for ribose utilization, and that its transcription is likely regulated by a LacI-type regulator encoded by rbsR, located immediately upstream of rbsA. Gel mobility shift assays using purified RbsRHis indicate that the promoter upstream of rbsABCDK is negatively controlled by RbsRHis binding to an 18 bp inverted repeat and that RbsR His binding activity is modulated by D-ribose. The rbsK gene of the rbs operon of B. breve UCC2003 was shown to specify a ribokinase (EC 2.7.1.15), which specifically directs its phosphorylating activity towards D-ribose, converting this pentose sugar to ribose-5-phosphate.

AB - Growth of Bifidobacterium breve UCC2003 on ribose leads to the transcriptional induction of the rbsACBDK gene cluster. Generation and phenotypic analysis of an rbsA insertion mutant established that the rbs gene cluster is essential for ribose utilization, and that its transcription is likely regulated by a LacI-type regulator encoded by rbsR, located immediately upstream of rbsA. Gel mobility shift assays using purified RbsRHis indicate that the promoter upstream of rbsABCDK is negatively controlled by RbsRHis binding to an 18 bp inverted repeat and that RbsR His binding activity is modulated by D-ribose. The rbsK gene of the rbs operon of B. breve UCC2003 was shown to specify a ribokinase (EC 2.7.1.15), which specifically directs its phosphorylating activity towards D-ribose, converting this pentose sugar to ribose-5-phosphate.

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DO - 10.1111/j.1751-7915.2009.00152.x

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JO - Microbial Biotechnology

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Ribose utilization by the human commensal Bifidobacterium breve UCC2003

Abstract

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