Sa1797 Altered Immunometabolism As a Result of Colonic Inflammation and Westernized Diet in Experimental Models of Colitis and Colitis-Associated Colorectal Cancer

Caitlin O'Mahony; Aisling M. Gavin; Aine Fanning; Ruth E. Bourke; Elaine Patterson; Rebecca Wall; Catherine Stanton; Fergus Shanahan; Silvia Melgar

doi:10.1016/s0016-5085(15)31113-6

Sa1797 Altered Immunometabolism As a Result of Colonic Inflammation and Westernized Diet in Experimental Models of Colitis and Colitis-Associated Colorectal Cancer

Caitlin O'Mahony
, Aisling M. Gavin
, Aine Fanning
, Ruth E. Bourke
, Elaine Patterson
, Rebecca Wall
, Catherine Stanton
, Fergus Shanahan
, Silvia Melgar

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Environmental factors, e.g. diet, immune dysregulation and genetic predisposition are thought to trigger inflammatory bowel diseases (IBD). Westernized diets have a negative impact on immunometabolism resulting in obesity, diabetes. The impact of IBD and westernized diet on immunometabolism are not fully explored. Aims: To examine the immunometabolic responses in experimental Colitis and Colitis-Association Cancer (CAC) and upon westernized diet feeding. Methods: Male C57BL/6 mice were fed low fat (LF, 10%Kcal) or high fat (HF, 45%Kcal) diet for 5 weeks. Colitis was induced by Dextran Sodium Sulphate (acute: 2%DSS-5d & water-4d; chronic: 1.5%DSS-5d & water-14d). CAC was induced by injection of the carcinogen azoxymethane accompanied by 3xDSS cycles. Plasma, visceral adipose tissue (VAT), liver and colons were harvested and assayed for inflammatory and metabolic markers. VAT stromal vascular cells (SVCs) were isolated and characterized by flow cytometry. Gas-liquid chromatography was employed to profile liver and VAT fatty acids (FA). Results: Mice with colitis and CAC presented significantly higher signs of disease (colon pro-inflammatory cytokines and tumor incidence), which were significantly reduced by HF-feeding (p

Original language	English (Ireland)
Pages (from-to)	S-335
Journal	Gastroenterology
Volume	148
Issue number	4
DOIs	https://doi.org/10.1016/s0016-5085(15)31113-6
Publication status	Published - Apr 2015

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10.1016/s0016-5085(15)31113-6

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O'Mahony, C., Gavin, A. M., Fanning, A., Bourke, R. E., Patterson, E., Wall, R., Stanton, C., Shanahan, F., & Melgar, S. (2015). Sa1797 Altered Immunometabolism As a Result of Colonic Inflammation and Westernized Diet in Experimental Models of Colitis and Colitis-Associated Colorectal Cancer. Gastroenterology, 148(4), S-335. https://doi.org/10.1016/s0016-5085(15)31113-6

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title = "Sa1797 Altered Immunometabolism As a Result of Colonic Inflammation and Westernized Diet in Experimental Models of Colitis and Colitis-Associated Colorectal Cancer",

abstract = "Background: Environmental factors, e.g. diet, immune dysregulation and genetic predisposition are thought to trigger inflammatory bowel diseases (IBD). Westernized diets have a negative impact on immunometabolism resulting in obesity, diabetes. The impact of IBD and westernized diet on immunometabolism are not fully explored. Aims: To examine the immunometabolic responses in experimental Colitis and Colitis-Association Cancer (CAC) and upon westernized diet feeding. Methods: Male C57BL/6 mice were fed low fat (LF, 10\%Kcal) or high fat (HF, 45\%Kcal) diet for 5 weeks. Colitis was induced by Dextran Sodium Sulphate (acute: 2\%DSS-5d \& water-4d; chronic: 1.5\%DSS-5d \& water-14d). CAC was induced by injection of the carcinogen azoxymethane accompanied by 3xDSS cycles. Plasma, visceral adipose tissue (VAT), liver and colons were harvested and assayed for inflammatory and metabolic markers. VAT stromal vascular cells (SVCs) were isolated and characterized by flow cytometry. Gas-liquid chromatography was employed to profile liver and VAT fatty acids (FA). Results: Mice with colitis and CAC presented significantly higher signs of disease (colon pro-inflammatory cytokines and tumor incidence), which were significantly reduced by HF-feeding (p",

author = "Caitlin O'Mahony and Gavin, \{Aisling M.\} and Aine Fanning and Bourke, \{Ruth E.\} and Elaine Patterson and Rebecca Wall and Catherine Stanton and Fergus Shanahan and Silvia Melgar",

year = "2015",

month = apr,

doi = "10.1016/s0016-5085(15)31113-6",

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O'Mahony, C, Gavin, AM, Fanning, A, Bourke, RE, Patterson, E, Wall, R, Stanton, C, Shanahan, F & Melgar, S 2015, 'Sa1797 Altered Immunometabolism As a Result of Colonic Inflammation and Westernized Diet in Experimental Models of Colitis and Colitis-Associated Colorectal Cancer', Gastroenterology, vol. 148, no. 4, pp. S-335. https://doi.org/10.1016/s0016-5085(15)31113-6

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AU - O'Mahony, Caitlin

AU - Gavin, Aisling M.

AU - Fanning, Aine

AU - Bourke, Ruth E.

AU - Patterson, Elaine

AU - Wall, Rebecca

AU - Stanton, Catherine

AU - Shanahan, Fergus

AU - Melgar, Silvia

PY - 2015/4

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N2 - Background: Environmental factors, e.g. diet, immune dysregulation and genetic predisposition are thought to trigger inflammatory bowel diseases (IBD). Westernized diets have a negative impact on immunometabolism resulting in obesity, diabetes. The impact of IBD and westernized diet on immunometabolism are not fully explored. Aims: To examine the immunometabolic responses in experimental Colitis and Colitis-Association Cancer (CAC) and upon westernized diet feeding. Methods: Male C57BL/6 mice were fed low fat (LF, 10%Kcal) or high fat (HF, 45%Kcal) diet for 5 weeks. Colitis was induced by Dextran Sodium Sulphate (acute: 2%DSS-5d & water-4d; chronic: 1.5%DSS-5d & water-14d). CAC was induced by injection of the carcinogen azoxymethane accompanied by 3xDSS cycles. Plasma, visceral adipose tissue (VAT), liver and colons were harvested and assayed for inflammatory and metabolic markers. VAT stromal vascular cells (SVCs) were isolated and characterized by flow cytometry. Gas-liquid chromatography was employed to profile liver and VAT fatty acids (FA). Results: Mice with colitis and CAC presented significantly higher signs of disease (colon pro-inflammatory cytokines and tumor incidence), which were significantly reduced by HF-feeding (p

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Sa1797 Altered Immunometabolism As a Result of Colonic Inflammation and Westernized Diet in Experimental Models of Colitis and Colitis-Associated Colorectal Cancer

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