Shedding light on betL*: pPL2-lux mediated real-time analysis of betL* expression in Listeria monocytogenes

Shauna M. Keane; Eamonn P. Culligan; Roland F. Hoffmann; Cormac G.M. Gahan; Colin Hill; William J. Snelling; Roy D. Sleator

doi:10.1080/21655979.2016.1171438

Shedding light on betL: pPL2-lux mediated real-time analysis of betL expression in Listeria monocytogenes

Shauna M. Keane
, Eamonn P. Culligan
, Roland F. Hoffmann
, Cormac G.M. Gahan
, Colin Hill
, William J. Snelling
, Roy D. Sleator

Munster Technological University

Research output: Contribution to journal › Comment/Debate

Abstract

We propose a mechanism of action for the betL* mutation which is based on DNA topology. Removing a single thymine residue from the betL σ(A) promoter's -10 and -35 spacer results in a 'twist'-mediated activation of transcription which accounts for the osmotolerance phenotype observed for strains expressing betL*.

Original language	English
Pages (from-to)	116-119
Number of pages	4
Journal	Bioengineered
Volume	7
Issue number	2
DOIs	https://doi.org/10.1080/21655979.2016.1171438
Publication status	Published - 2 Apr 2016
Externally published	Yes

Keywords

betL*
Listeria
lux
osmotolerance

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10.1080/21655979.2016.1171438

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title = "Shedding light on betL*: pPL2-lux mediated real-time analysis of betL* expression in Listeria monocytogenes",

abstract = "We propose a mechanism of action for the betL* mutation which is based on DNA topology. Removing a single thymine residue from the betL σ(A) promoter's -10 and -35 spacer results in a 'twist'-mediated activation of transcription which accounts for the osmotolerance phenotype observed for strains expressing betL*.",

keywords = "betL*, Listeria, lux, osmotolerance",

author = "Keane, \{Shauna M.\} and Culligan, \{Eamonn P.\} and Hoffmann, \{Roland F.\} and Gahan, \{Cormac G.M.\} and Colin Hill and Snelling, \{William J.\} and Sleator, \{Roy D.\}",

year = "2016",

month = apr,

day = "2",

doi = "10.1080/21655979.2016.1171438",

language = "English",

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pages = "116--119",

journal = "Bioengineered",

issn = "2165-5979",

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T1 - Shedding light on betL*

T2 - pPL2-lux mediated real-time analysis of betL* expression in Listeria monocytogenes

AU - Keane, Shauna M.

AU - Culligan, Eamonn P.

AU - Hoffmann, Roland F.

AU - Gahan, Cormac G.M.

AU - Hill, Colin

AU - Snelling, William J.

AU - Sleator, Roy D.

PY - 2016/4/2

Y1 - 2016/4/2

N2 - We propose a mechanism of action for the betL* mutation which is based on DNA topology. Removing a single thymine residue from the betL σ(A) promoter's -10 and -35 spacer results in a 'twist'-mediated activation of transcription which accounts for the osmotolerance phenotype observed for strains expressing betL*.

AB - We propose a mechanism of action for the betL* mutation which is based on DNA topology. Removing a single thymine residue from the betL σ(A) promoter's -10 and -35 spacer results in a 'twist'-mediated activation of transcription which accounts for the osmotolerance phenotype observed for strains expressing betL*.

KW - betL

KW - Listeria

KW - lux

KW - osmotolerance

UR - https://www.scopus.com/pages/publications/85021853759

U2 - 10.1080/21655979.2016.1171438

DO - 10.1080/21655979.2016.1171438

M3 - Comment/Debate

C2 - 27212260

AN - SCOPUS:85021853759

SN - 2165-5979

VL - 7

SP - 116

EP - 119

JO - Bioengineered

JF - Bioengineered

IS - 2

ER -

Shedding light on betL: pPL2-lux mediated real-time analysis of betL expression in Listeria monocytogenes

Abstract

Keywords

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